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. 1982 Apr;43(4):740–746. doi: 10.1128/aem.43.4.740-746.1982

Evaluation of cell lines and immunofluorescence and plaque assay procedures for quantifying reoviruses in sewage.

D N Ridinger, R S Spendlove, B B Barnett, D B George, J C Roth
PMCID: PMC241911  PMID: 7044308

Abstract

Twelve continuous cell lines were tested to determine their sensitivity to reovirus types 1, 2, and 3 isolated from sewage. Madin-Darby bovine kidney (MDBK), rhesus monkey kidney (LLC-MK2), and human embryonic intestinal (intestinal 407) cells were most sensitive, respectively. In a similar study, MDBK cells were more sensitive than LLC-MK2 and Buffalo green monkey kidney (BGM) cells to sewage-isolated, protamine-precipitated reoviruses which had not been serotyped and had no previous cell contact. Sewage-isolated, protamine-precipitated reoviruses were also used in conjunction with MDBK cells in a comparative evaluation of immunofluorescent cell count and plaque assay procedures. The immunofluorescence assay is more sensitive and more rapid than the plaque assay. Reoviruses in excess of 10(4)/liter of raw sewage were detected by the immunofluorescent cell count assay.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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