Abstract
The major objective of this study was to describe the mechanism(s) of cyclic AMP uptake by natural populations of marine bacteria. A second objective was to determine whether this uptake could contribute to the intracellular regulatory pool of cyclic AMP. Using high-specific-activity 32P-labeled cyclic AMP, we found several high-affinity uptake systems. The highest-affinity system had a half-saturation constant of <10 pM. This system was extremely specific for cyclic nucleotides, particularly cyclic AMP. It appeared to meet the criteria for active transport. Uptake of cyclic AMP over a wide concentration range (up to 2 μM) showed multiphasic kinetics, with half-saturation constants of 1 nM and greater. These lower-affinity systems were much less specific for cyclic nucleotides. Although much of the labeled cyclic AMP taken up by the high-affinity systems was metabolized, some remained as intact cyclic AMP within the cells during 1 h of incubation. This suggests that at least some of the bacteria use cyclic AMP dissolved in seawater to augment their intracellular pools.
Full text
PDF







Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brunton L. L., Buss J. E. Export of cyclic AMP by mammalian reticulocytes. J Cyclic Nucleotide Res. 1980;6(5):369–377. [PubMed] [Google Scholar]
- Butcher R. W. Cyclic 3',5'-nucleotide phosphodiesterase from bovine heart. Methods Enzymol. 1974;38:218–223. doi: 10.1016/0076-6879(74)38035-4. [DOI] [PubMed] [Google Scholar]
- CARLUCCI A. F., PRAMER D. Factors influencing the plate method for determining abundance of bacteria in sea water. Proc Soc Exp Biol Med. 1957 Nov;96(2):392–394. doi: 10.3181/00379727-96-23487. [DOI] [PubMed] [Google Scholar]
- Fuhrman J. A., Azam F. Bacterioplankton secondary production estimates for coastal waters of british columbia, antarctica, and california. Appl Environ Microbiol. 1980 Jun;39(6):1085–1095. doi: 10.1128/aem.39.6.1085-1095.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gerisch G., Malchow D., Roos W., Wick U. Oscillations of cyclic nucleotide concentrations in relation to the excitability of Dictyostelium cells. J Exp Biol. 1979 Aug;81:33–47. doi: 10.1242/jeb.81.1.33. [DOI] [PubMed] [Google Scholar]
- Goldenbaum P. E., Hall G. A. Transport of cyclic adenosine 3',5'-monophosphate across Escherichia coli vesicle membranes. J Bacteriol. 1979 Nov;140(2):459–467. doi: 10.1128/jb.140.2.459-467.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hodson R. E., Azam F. Occurrence and Characterization of a Phosphoenolpyruvate: Glucose Phosphotransferase System in a Marine Bacterium, Serratia marinorubra. Appl Environ Microbiol. 1979 Dec;38(6):1086–1091. doi: 10.1128/aem.38.6.1086-1091.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Joseph E., Danchin A., Ullmann A. Regulation of galactose operon expression: glucose effects and role of cyclic adenosine 3',5'-monophosphate. J Bacteriol. 1981 Apr;146(1):149–154. doi: 10.1128/jb.146.1.149-154.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lefebvre G., Martin N., Gay R. Periodic cyclic AMP uptake by synchronously grown cells of Nocardia restricta and Arthrobacter globiformis. FEBS Lett. 1978 Sep 1;93(1):55–57. doi: 10.1016/0014-5793(78)80803-5. [DOI] [PubMed] [Google Scholar]
- Levitzki A., Koshland D. E., Jr Negative cooperativity in regulatory enzymes. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1121–1128. doi: 10.1073/pnas.62.4.1121. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McKay D. B., Steitz T. A. Structure of catabolite gene activator protein at 2.9 A resolution suggests binding to left-handed B-DNA. Nature. 1981 Apr 30;290(5809):744–749. doi: 10.1038/290744a0. [DOI] [PubMed] [Google Scholar]
- Nealson K. H., Hastings J. W. Bacterial bioluminescence: its control and ecological significance. Microbiol Rev. 1979 Dec;43(4):496–518. doi: 10.1128/mr.43.4.496-518.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pastan I. H., Johnson G. S., Anderson W. B. Role of cyclic nucleotides in growth control. Annu Rev Biochem. 1975;44:491–522. doi: 10.1146/annurev.bi.44.070175.002423. [DOI] [PubMed] [Google Scholar]
- Pastan I., Adhya S. Cyclic adenosine 5'-monophosphate in Escherichia coli. Bacteriol Rev. 1976 Sep;40(3):527–551. doi: 10.1128/br.40.3.527-551.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rickenberg H. V. Cyclic AMP in prokaryotes. Annu Rev Microbiol. 1974;28(0):353–369. doi: 10.1146/annurev.mi.28.100174.002033. [DOI] [PubMed] [Google Scholar]
- Saier M. H., Jr, Feucht B. U., McCaman M. T. Regulation of intracellular adenosine cyclic 3':5'-monophosphate levels in Escherichia coli and Salmonella typhimurium. Evidence for energy-dependent excretion of the cyclic nucleotide. J Biol Chem. 1975 Oct 10;250(19):7593–7601. [PubMed] [Google Scholar]
- Taniguchi T., O'Neill M., de Crombrugghe B. Interaction site of Escherichia coli cyclic AMP receptor protein on DNA of galactose operon promoters. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5090–5094. doi: 10.1073/pnas.76.10.5090. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tao M. Preparation and properties of adenylate cyclase from Escherichia coli. Methods Enzymol. 1974;38:155–160. doi: 10.1016/0076-6879(74)38024-x. [DOI] [PubMed] [Google Scholar]
- Tsukamoto T., Suyama K., Germann P., Sonenberg M. Adenosine cyclic 3',5'-monophosphate uptake and regulation of membrane protein kinase in intact human erythrocytes. Biochemistry. 1980 Mar 4;19(5):918–924. doi: 10.1021/bi00546a015. [DOI] [PubMed] [Google Scholar]
- Ullmann A., Joseph E., Danchin A. Cyclic AMP as a modulator of polarity in polycistronic transcriptional units. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3194–3197. doi: 10.1073/pnas.76.7.3194. [DOI] [PMC free article] [PubMed] [Google Scholar]