Abstract
An enzyme immunoassay was developed to detect Salmonella in foods. Indirect test protocols were developed for use with microtitration plates or Gilford microcuvettes. Samples from enrichment cultures were mixed with H-specific immunoglobulin G and allowed to react; unbound antibody was removed by three 5-min centrifugation washes; goat anti-rabbit antibody conjugated to alkaline phosphatase was added and allowed to react; and unbound conjugate was removed by centrifugation washing as before. Salmonella-positive samples were indicated by the production of a chromogenic reaction product after the addition of alkaline phosphatase substrate. The color could be read visually or quantified by absorbance. Ninety-eight food samples were examined to compare the enzyme immunoassay with enrichment serology, immunofluorescence, and the Food and Drug Administration pure culture technique. The enzyme immunoassay was sensitive and specific, and it possessed advantages over methods currently in use. Furthermore, when the enzyme immunoassay was used to screen preenrichment media, the results indicated that it might be decidedly more sensitive than the conventional pure culture technique.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ADA G. L., NOSSAL G. J., PYE J., ABBOT A. ANTIGENS IN IMMUNITY. I. PREPARATION AND PROPERTIES OF FLAGELLAR ANTIGENS FROM SALMONELLA ADELAIDE. Aust J Exp Biol Med Sci. 1964 Jun;42:267–282. [PubMed] [Google Scholar]
- Krysinski E. P., Heimsch R. C. Use of enzyme-labeled antibodies to detect Salmonella in foods. Appl Environ Microbiol. 1977 Apr;33(4):947–954. doi: 10.1128/aem.33.4.947-954.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Landy M., Sanderson R. P., Jackson A. L. Humoral and cellular aspects of the immune response to the somatic antigen of Salmonella enteritidis. J Exp Med. 1965 Sep 1;122(3):483–504. doi: 10.1084/jem.122.3.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
- March S. C., Parikh I., Cuatrecasas P. A simplified method for cyanogen bromide activation of agarose for affinity chromatography. Anal Biochem. 1974 Jul;60(1):149–152. doi: 10.1016/0003-2697(74)90139-0. [DOI] [PubMed] [Google Scholar]
- NOSSAL G. J., ADA G. L., AUSTIN C. M. ANTIGENS IN IMMUNITY. II. IMMUNOGENIC PROPERTIES OF FLAGELLA, POLYMERIZED FLAGELLIN AND FLAGELLIN IN THE PRIMARY RESPONSE. Aust J Exp Biol Med Sci. 1964 Jun;42:283–294. [PubMed] [Google Scholar]
- Polin R. A., Kennett R. Use of monoclonal antibodies in an enzyme immunoassay for rapid identification of group B Streptococcus types II and III. J Clin Microbiol. 1980 Apr;11(4):332–336. doi: 10.1128/jcm.11.4.332-336.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sperber W. H., Deibel R. H. Accelerated procedure for Salmonella detection in dried foods and feeds involving only borth cultures and serological reactions. Appl Microbiol. 1969 Apr;17(4):533–539. doi: 10.1128/am.17.4.533-539.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Swaminathan B., Ayres J. C., Williams J. E. Control of nonspecific staining in the fluorescent antibody technique for the detection of salmonellae in foods. Appl Environ Microbiol. 1978 May;35(5):911–919. doi: 10.1128/aem.35.5.911-919.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yolken R. H., Stopa P. J. Enzyme-linked fluorescence assay: Ultrasensitive solid-phase assay for detection of human rotavirus. J Clin Microbiol. 1979 Sep;10(3):317–321. doi: 10.1128/jcm.10.3.317-321.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]