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. 1982 Jul;44(1):246–249. doi: 10.1128/aem.44.1.246-249.1982

Plasmid Involvement in Parathion Hydrolysis by Pseudomonas diminuta

Cüneyt M Serdar 1, David T Gibson 1, Douglas M Munnecke 1,†, John H Lancaster 1
PMCID: PMC241997  PMID: 16346063

Abstract

An organism identified as Pseudomonas diminuta was found to hydrolyze parathion. Cells grown for 48 h contained 3,400 U of parathion hydrolase activity per liter of broth. Expression of enzymatic activity was lost at a high frequency (9 to 12%) after treatment with mitomycin C. Hydrolase-negative derivatives were missing a plasmid present in the wild-type organism. The molecular mass of this plasmid (pCS1), as determined by electron microscopy, was about 44 × 106 daltons.

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Selected References

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