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. 1982 Aug;44(2):317–320. doi: 10.1128/aem.44.2.317-320.1982

Development of a shaker culture of Buffalo green monkey kidney cells: potential use for detection of enteroviruses.

G Goldstein, L E Guskey
PMCID: PMC242012  PMID: 6289745

Abstract

Buffalo green monkey kidney cells were adapted to grow as shaker cultures. Replication of environmental and clinical isolates of poliovirus, coxsackievirus, and echovirus in these cultures was analyzed by plaque assay and compared with replication in Buffalo green monkey kidney cell monolayers and HEp-2 cell shaker cultures. Dose-response tests with various concentrations of Mahoney type 1 poliovirus indicated that Buffalo green monkey kidney cell shaker cultures could detect as little as 1 PFU in an inoculum of 0.2 ml. These data suggest that Buffalo green monkey kidney cell shaker cultures can be effectively used for the detection of small quantities of enteroviruses from environmental sources.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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