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. 1982 Aug;44(2):383–394. doi: 10.1128/aem.44.2.383-394.1982

Fate of virus in wastewater applied to slow-infiltration land treatment systems.

S A Schaub, H T Bausum, G W Taylor
PMCID: PMC242023  PMID: 6289746

Abstract

The removal of seeded coliphage f2 and indigenous enteroviruses from primary and secondary wastewaters applied by spray irrigation to sandy loam and silt loam soils in field test cells was examined. The amount of f2 recovered from 170-cm-deep soil percolate samples taken over a 53-day period never exceeded 0.1% of applied virus levels and was usually below detection limits. Indigenous enterovirus levels in percolate waters also constituted only a small portion of those found in the wastewaters. At 10 days after seeding, f2 virus was present throughout the soil column but tended to accumulate around the soil core middepths. Coliphage f2 disappeared from the soil surface regions at a high rate, and by 53 days very little virus could be detected within the length of the soil columns. Sterilized soil core segments from different depths were studied to determine their virus adsorption capabilities when suspended in either wastewater, test cell percolate water, or distilled water containing divalent cations. The adsorptive capacity of Windsor and Charlton soils for poliovirus 1 and coliphage f2 increased greatly with the soil sample depth until leveling off at the midcore depths. Soil suspended in wastewater had the least virus adsorption capability for all depths studied.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Duboise S. M., Moore B. E., Sagik B. P. Poliovirus survival and movement in a sandy forest soil. Appl Environ Microbiol. 1976 Apr;31(4):536–543. doi: 10.1128/aem.31.4.536-543.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Enger M. D., Stubbs E. A., Mitra S., Kaesberg P. BIOPHYSICAL CHARACTERISTICS OF THE RNA-CONTAINING BACTERIAL VIRUS R17. Proc Natl Acad Sci U S A. 1963 Jun;49(6):857–860. doi: 10.1073/pnas.49.6.857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gerba C. P., Lance J. C. Poliovirus removal from primary and secondary sewage effluent by soil filtration. Appl Environ Microbiol. 1978 Aug;36(2):247–251. doi: 10.1128/aem.36.2.247-251.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gilbert R. G., Gerba C. P., Rice R. C., Bouwer H., Wallis C., Melnick J. L. Virus and bacteria removal from wastewater by land treatment. Appl Environ Microbiol. 1976 Sep;32(3):333–338. doi: 10.1128/aem.32.3.333-338.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kim C. K., Gentile D. M., Sproul O. J. Mechanism of Ozone Inactivation of Bacteriophage f2. Appl Environ Microbiol. 1980 Jan;39(1):210–218. doi: 10.1128/aem.39.1.210-218.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LOEB T., ZINDER N. D. A bacteriophage containing RNA. Proc Natl Acad Sci U S A. 1961 Mar 15;47:282–289. doi: 10.1073/pnas.47.3.282. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Schaub S. A., Kenyon K. F., Bledsoe B., Thomas R. E. Evaluation of the overland runoff mode of land wastewater treatment for virus removal. Appl Environ Microbiol. 1980 Jan;39(1):127–134. doi: 10.1128/aem.39.1.127-134.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Schaub S. A., Sagik B. P. Association of enteroviruses with natural and artificially introduced colloidal solids in water and infectivity of solids-associated virions. Appl Microbiol. 1975 Aug;30(2):212–222. doi: 10.1128/am.30.2.212-222.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Schaub S. A., Sorber C. A. Virus and bacteria removal from wastewater by rapid infiltration through soil. Appl Environ Microbiol. 1977 Mar;33(3):609–619. doi: 10.1128/aem.33.3.609-619.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Vaughn J. M., Landry E. F., Baranosky L. J., Beckwith C. A., Dahl M. C., Delihas N. C. Survey of human virus occurrence in wastewater-recharged groundwater on Long Island. Appl Environ Microbiol. 1978 Jul;36(1):47–51. doi: 10.1128/aem.36.1.47-51.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Wellings F. M., Lewis A. L., Mountain C. W., Pierce L. V. Demonstration of virus in groundwater after effluent discharge onto soil. Appl Microbiol. 1975 Jun;29(6):751–757. doi: 10.1128/am.29.6.751-757.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

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