Abstract
Cultures of rumen bacteria can be stored at −20°C for at least 2 years in a liquid medium containing 20% glycerol. Thawing, sampling, and refreezing do not significantly affect viability.
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Selected References
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- Bryant M. P., Robinson I. M. Some Nutritional Requirements of the Genus Ruminococcus. Appl Microbiol. 1961 Mar;9(2):91–95. doi: 10.1128/am.9.2.91-95.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bryant M. P., Robinson I. M. Studies on the Nitrogen Requirements of Some Ruminal Cellulolytic Bacteria. Appl Microbiol. 1961 Mar;9(2):96–103. doi: 10.1128/am.9.2.96-103.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HUNGATE R. E. The anaerobic mesophilic cellulolytic bacteria. Bacteriol Rev. 1950 Mar;14(1):1–49. doi: 10.1128/br.14.1.1-49.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Macy J. M., Snellen J. E., Hungate R. E. Use of syringe methods for anaerobiosis. Am J Clin Nutr. 1972 Dec;25(12):1318–1323. doi: 10.1093/ajcn/25.12.1318. [DOI] [PubMed] [Google Scholar]
- Miller T. L., Wolin M. J. A serum bottle modification of the Hungate technique for cultivating obligate anaerobes. Appl Microbiol. 1974 May;27(5):985–987. doi: 10.1128/am.27.5.985-987.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]