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. 1982 Sep;44(3):747–750. doi: 10.1128/aem.44.3.747-750.1982

Improved Method for Detection of Starch Hydrolysis

Motiram R Dhawale 1, J Jeffrey Wilson 1,†, George G Khachatourians 1, W Mike Ingledew 1
PMCID: PMC242086  PMID: 16346102

Abstract

A new starch hydrolysis detection method which does not rely on iodine staining or the use of color-complexed starch is described. A linear relationship was obtained with agar-starch plates when net clearing zones around colonies of yeasts were plotted against enzyme levels (semilogarithm scale) produced by the same yeast strains in liquid medium. A similar relationship between starch clearing zones and α-amylase levels from three different sources was observed. These observations suggest that the method is useful in mutant isolations, strain improvement programs, and the prediction of α-amylase activities in culture filtrates or column effluents.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ceska M. Enzymatic catalysis in solidified media. Eur J Biochem. 1971 Sep 24;22(2):186–192. doi: 10.1111/j.1432-1033.1971.tb01531.x. [DOI] [PubMed] [Google Scholar]
  2. Ceska M., Hultman E., Ingelman B. G. A new method for determination of alpha-amylase. Experientia. 1969 May 15;25(5):555–556. doi: 10.1007/BF01900818. [DOI] [PubMed] [Google Scholar]
  3. Ceska M. Hydrolysis of a water insoluble substrate incorporated into solidified agar medium by enzyme -amylase contained in serum and urine of patients suffering from acute pancreatitis. Clin Chim Acta. 1972 Feb;36(2):463–471. doi: 10.1016/0009-8981(72)90021-6. [DOI] [PubMed] [Google Scholar]
  4. Iverson W. G., Millis N. F. A method for the detection of starch hydrolysis by bacteria. J Appl Bacteriol. 1974 Sep;37(3):443–446. doi: 10.1111/j.1365-2672.1974.tb00460.x. [DOI] [PubMed] [Google Scholar]
  5. Klein B., Foreman J. A., Searcy R. L. The synthesis and utilization of Cibachron Blue-amylose: a new chromogenic substrate for determination of amylase activity. Anal Biochem. 1969 Oct 1;31(1):412–425. doi: 10.1016/0003-2697(69)90283-8. [DOI] [PubMed] [Google Scholar]
  6. MACPHERSON M. J. Isolation and identification of amylolytic Streptococci from the rumen of the sheep. J Pathol Bacteriol. 1953 Jul;66(1):95–102. doi: 10.1002/path.1700660113. [DOI] [PubMed] [Google Scholar]
  7. McCleary B. V. New chromogenic substrates for the assay of alpha-amylase and (1 leads to 4)-beta-D-glucanase. Carbohydr Res. 1980 Nov 1;86(1):97–104. doi: 10.1016/s0008-6215(00)84584-x. [DOI] [PubMed] [Google Scholar]
  8. Mitchell W. D., Murchison L. E. The effect of clofibrate on serum and faecal lipids. Clin Chim Acta. 1972 Jan;36(1):153–161. doi: 10.1016/0009-8981(72)90171-4. [DOI] [PubMed] [Google Scholar]
  9. Rinderknecht H., Wilding P., Haverback B. J. A new method for the determination of alpha-amylase. Experientia. 1967 Oct 15;23(10):805–805. doi: 10.1007/BF02146851. [DOI] [PubMed] [Google Scholar]
  10. SEELEY H. W., DAIN J. A. Starch hydrolyzing streptococci. J Bacteriol. 1960 Feb;79:230–235. doi: 10.1128/jb.79.2.230-235.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Wilson J. J., Ingledew W. M. Isolation and characterization of Schwanniomyces alluvius amylolytic enzymes. Appl Environ Microbiol. 1982 Aug;44(2):301–307. doi: 10.1128/aem.44.2.301-307.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

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