Abstract
RNA was degraded at 60 degrees C for 24 h by halophilic nuclease H in supernatants from broth cultures of Micrococcus varians subsp. halophilus containing 12% NaCl. Since contaminating 5'-nucleotidase exhibited almost no activity under these conditions, the 5'-GMP formed could be recovered from the reaction mixture, and the yield was 805 mg from 5 g of RNA.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Kamekura M., Onishi H. A medium for commercial production of the halophilic Micrococcus nuclease. Can J Microbiol. 1979 Sep;25(9):1113–1116. doi: 10.1139/m79-172. [DOI] [PubMed] [Google Scholar]
- Kamekura M., Onishi H. Halophilic nuclease from a moderately halophilic Micrococcus varians. J Bacteriol. 1974 Aug;119(2):339–344. doi: 10.1128/jb.119.2.339-344.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kamekura M., Onishi H. Properties of the halophilic nuclease of a moderate halophile, Micrococcus varians subsp. halophilus. J Bacteriol. 1978 Jan;133(1):59–65. doi: 10.1128/jb.133.1.59-65.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]