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. 1982 Oct;44(4):996–1000. doi: 10.1128/aem.44.4.996-1000.1982

Isolation and Complementation Studies of Auxotrophic Mutants of the Lignin-Degrading Basidiomycete Phanerochaete chrysosporium

Michael H Gold 1, Therese M Cheng 1, Mary B Mayfield 1
PMCID: PMC242130  PMID: 16346124

Abstract

A variety of auxotrophic strains of Phanerochaete chrysosporium were isolated after treatment of conidia with UV and X rays. Complementation studies with these strains demonstrated heterokaryotic mycelia and conidia in this organism. Nuclear staining also showed that conidia can be mono-, di-, or multinucleate. Complementation tests allowed the separation of each auxotrophic class with the same phenotype into complementation groups.

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Selected References

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