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. 1982 Dec;44(6):1318–1324. doi: 10.1128/aem.44.6.1318-1324.1982

Acetone and Butanol Production by Clostridium acetobutylicum in a Synthetic Medium

Frédéric Monot 1, Jean-René Martin 1, Henri Petitdemange 1, Robert Gay 1
PMCID: PMC242190  PMID: 16346149

Abstract

The effect of the component concentrations of a synthetic medium on acetone and butanol fermentation by Clostridium acetobutylicum ATCC 824 was investigated. Cell growth was dependent on the presence of Mg, Fe, and K in the medium. Mg and Mn had deleterious effects when in excess. Ammonium acetate in excess caused acid fermentation. The metabolism was composed of two phases: an acid phase and a solvent one. Low concentrations of glucose allowed the first phase only. The theoretical ratio of the conversion of glucose to solvents, which was 28 to 33%, was obtained with the following medium: MgSO4, 50 to 200 mg/liter; MnSO4, 0 to 20 mg/liter; KCl, 0.015 to 8 g/liter (an equivalent concentration of K+ was supplied in the form of KH2PO4 and K2HPO4); FeSO4, 1 to 50 mg/liter; ammonium acetate, 1.1 to 2.2 g/liter; para-aminobenzoic acid, 1 mg/liter; biotin, 0.01 mg/liter; glucose, 20 to 60 g/liter.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barber J. M., Robb F. T., Webster J. R., Woods D. R. Bacteriocin production by Clostridium acetobutylicum in an industrial fermentation process. Appl Environ Microbiol. 1979 Mar;37(3):433–437. doi: 10.1128/aem.37.3.433-437.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DOI S., SUGAMA S. [Role of acetate in the nutrition of Clostridium, acetone and butyl alcohol producing strain No. 314]. C R Seances Soc Biol Fil. 1960;154:1687–1690. [PubMed] [Google Scholar]
  3. Davies R., Stephenson M. Studies on the acetone-butyl alcohol fermentation: Nutritional and other factors involved in the preparation of active suspensions of Cl. acetobutylicum (Weizmann). Biochem J. 1941 Dec;35(12):1320–1331. doi: 10.1042/bj0351320. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Davies R. Studies on the acetone-butyl alcohol fermentation: Intermediates in the fermentation of glucose by Cl. acetobutylicum. 3. Potassium as an essential factor in the fermentation of maize meal by Cl. acetobutylicum (BY). Biochem J. 1942 Sep;36(7-9):582–599. doi: 10.1042/bj0360582. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Petitdemange H., Cherrier C., Raval R., Gay R. Regulation of the NADH and NADPH-ferredoxin oxidoreductases in clostridia of the butyric group. Biochim Biophys Acta. 1976 Feb 24;421(2):334–337. doi: 10.1016/0304-4165(76)90300-7. [DOI] [PubMed] [Google Scholar]
  6. Petitdemange H., Marczak R., Blusson H., Gay R. Isolation and properties of reduced nicotinamide adenine dinucleotiderubredoxin oxidoreductase of Clostridium acetobutylicum. Biochem Biophys Res Commun. 1979 Dec 28;91(4):1258–1265. doi: 10.1016/0006-291x(79)91202-6. [DOI] [PubMed] [Google Scholar]
  7. ROSENFELD B., SIMON E. The mechanism of the butanol-acetone fermentation. I. The rôle of pyruvate as an intermediate. J Biol Chem. 1950 Sep;186(1):395–404. [PubMed] [Google Scholar]
  8. Zeikus J. G. Chemical and fuel production by anaerobic bacteria. Annu Rev Microbiol. 1980;34:423–464. doi: 10.1146/annurev.mi.34.100180.002231. [DOI] [PubMed] [Google Scholar]

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