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. 1983 Jan;45(1):187–192. doi: 10.1128/aem.45.1.187-192.1983

Sulfate Reducers Can Outcompete Methanogens at Freshwater Sulfate Concentrations †

Derek R Lovley 1,‡, Michael J Klug 1
PMCID: PMC242251  PMID: 16346164

Abstract

Acetate and hydrogen metabolism by sulfate reducers and methanogens in the profundal sediments of an oligotrophic lake were examined. Inhibition of sulfate reduction with molybdate stimulated methane production from both hydrogen and acetate. Molybdate did not stimulate methane production in sediments that were preincubated to deplete the sulfate pool. Sulfate reduction accounted for 30 to 81% of the total of terminal metabolism proceeding through sulfate reduction and methane production in Eckman grab samples of surface sediments. The ability of sulfate reducers to effectively compete with methanogens for acetate was related to the sulfate reducers' lower half-saturation constant for acetate metabolism at in situ sulfate concentrations. Processes other than sulfate reduction and methanogenesis consumed hydrogen at elevated hydrogen partial pressures and prevented a kinetic analysis of hydrogen uptake by sulfate reducers and methanogens. The demonstration that sulfate reducers can successfully compete with methanogens for hydrogen and acetate in sediments at in situ sulfate concentrations of 60 to 105 μM extends the known range of sediment habitats in which sulfate reduction can be a dominant terminal process.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abram J. W., Nedwell D. B. Hydrogen as a substrate for methanogenesis and sulphate reduction in anaerobic saltmarsh sediment. Arch Microbiol. 1978 Apr 27;117(1):93–97. doi: 10.1007/BF00689357. [DOI] [PubMed] [Google Scholar]
  2. Cappenberg T. E. Interrelations between sulfate-reducing and methane-producing bacteria in bottom deposits of a fresh-water lake. II. Inhibition experiments. Antonie Van Leeuwenhoek. 1974;40(2):297–306. doi: 10.1007/BF00394388. [DOI] [PubMed] [Google Scholar]
  3. Ingvorsen K., Zeikus J. G., Brock T. D. Dynamics of bacterial sulfate reduction in a eutrophic lake. Appl Environ Microbiol. 1981 Dec;42(6):1029–1036. doi: 10.1128/aem.42.6.1029-1036.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Laanbroek H. J., Pfennig N. Oxidation of short-chain fatty acids by sulfate-reducing bacteria in freshwater and in marine sediments. Arch Microbiol. 1981 Jan;128(3):330–335. doi: 10.1007/BF00422540. [DOI] [PubMed] [Google Scholar]
  5. Lovley D. R., Dwyer D. F., Klug M. J. Kinetic analysis of competition between sulfate reducers and methanogens for hydrogen in sediments. Appl Environ Microbiol. 1982 Jun;43(6):1373–1379. doi: 10.1128/aem.43.6.1373-1379.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lovley D. R., Klug M. J. Intermediary metabolism of organic matter in the sediments of a eutrophic lake. Appl Environ Microbiol. 1982 Mar;43(3):552–560. doi: 10.1128/aem.43.3.552-560.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Smith R. L., Klug M. J. Electron donors utilized by sulfate-reducing bacteria in eutrophic lake sediments. Appl Environ Microbiol. 1981 Jul;42(1):116–121. doi: 10.1128/aem.42.1.116-121.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Smith R. L., Klug M. J. Reduction of sulfur compounds in the sediments of a eutrophic lake basin. Appl Environ Microbiol. 1981 May;41(5):1230–1237. doi: 10.1128/aem.41.5.1230-1237.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sørensen J., Christensen D., Jørgensen B. B. Volatile Fatty acids and hydrogen as substrates for sulfate-reducing bacteria in anaerobic marine sediment. Appl Environ Microbiol. 1981 Jul;42(1):5–11. doi: 10.1128/aem.42.1.5-11.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Thauer R. K., Jungermann K., Decker K. Energy conservation in chemotrophic anaerobic bacteria. Bacteriol Rev. 1977 Mar;41(1):100–180. doi: 10.1128/br.41.1.100-180.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Winfrey M. R., Zeikus J. G. Effect of sulfate on carbon and electron flow during microbial methanogenesis in freshwater sediments. Appl Environ Microbiol. 1977 Feb;33(2):275–281. doi: 10.1128/aem.33.2.275-281.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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