Abstract
A new strain of Prototheca zopfii Krüger was grown on acetate or on pure n-alkanes. A maximum acetate-supported exponential growth of 12 divisions day−1 occurred at pH 5 and 30°C. At 25°C, growth on n-alkanes was almost as fast, but no growth occurred at 30°C. After 4 days at 25°C, 34 to 45% of the n-alkanes had been removed, whereas at 21°C and slower growth, utilization was twofold greater after 15 days. Rates of growth and utilization increased markedly after a point of sudden emulsification.
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