Abstract
Rosamicin is one of the important macrolide antibiotics that has clinical efficacy and broad-spectrum antibacterial activity. Using a mutant strain of Micromonospora rosaria (NRRL 3718), a chemically defined medium was developed, and some fermentation conditions that are important to rosamicin biosynthesis were optimized to achieve rosamicin productivity of 230 μg/ml. Soluble starch and l-asparagine were found to be the best carbon and nitrogen sources, and a stimulative effect of magnesium and zinc ions was also found. The medium developed contains: soluble starch, 4%; l-asparagine, 0.15%; K2HPO4, 0.075%; CaCO3, 0.6%; MgSO4 · 7H2O, 0.05%; FeSO4 · 7H2O, 10−4 M; CuSO4 · 5H2O, 10−5 M; ZnSO4 · 7H2O, 10−5 M; and MnSO4 · (4-6)H2O, 10−6 M. The required air supply was about 40 mmol of O2 liter−1 · h−1 · atm−1, and the favorable culture temperature was 28 to 29°C.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Drew S. W., Demain A. L. Effect of primary metabolites on secondary metabolism. Annu Rev Microbiol. 1977;31:343–356. doi: 10.1146/annurev.mi.31.100177.002015. [DOI] [PubMed] [Google Scholar]
- Ganguly A. K., Lee B. K., Brambilla R., Condon R., Sarre O. Biosynthesis of rosamicin. J Antibiot (Tokyo) 1976 Sep;29(9):976–977. doi: 10.7164/antibiotics.29.976. [DOI] [PubMed] [Google Scholar]
- Martin J. F., Demain A. L. Control of antibiotic biosynthesis. Microbiol Rev. 1980 Jun;44(2):230–251. doi: 10.1128/mr.44.2.230-251.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Martin J. F., McDaniel L. E. Production of polyene macrolide antibiotics. Adv Appl Microbiol. 1977;21:1–52. doi: 10.1016/s0065-2164(08)70037-6. [DOI] [PubMed] [Google Scholar]
- Polsinelli M., Beretta M. Genetic Recombination in Crosses Between Streptomyces aureofaciens and Streptomyces rimosus. J Bacteriol. 1966 Jan;91(1):63–68. doi: 10.1128/jb.91.1.63-68.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Porter J. N. Cultural conditions for antibiotic-producing microorganisms. Methods Enzymol. 1975;43:3–23. doi: 10.1016/0076-6879(75)43077-4. [DOI] [PubMed] [Google Scholar]
- Wagman G. H., Waitz J. A., Marquez J., Murawaski A., Oden E. M., Testa R. T., Weinstein M. J. A new Micromonospora-produced macrolide antibiotic, rosamicin. J Antibiot (Tokyo) 1972 Nov;25(11):641–646. doi: 10.7164/antibiotics.25.641. [DOI] [PubMed] [Google Scholar]