Abstract
A study was made to determine the effects of temperature and moisture on the D-value of a common biological indicator. Relative humidity (RH) was varied between 10 and 70% in increments of 10%, and temperature was varied between 30 and 70 degrees C in increments of 10 degrees C. Temperature was found to have a pronounced effect on the D-value. At 60% RH, the D-value varied from 15.0 min at 30 degrees C to 1.1 min at 70 degrees C. When RH was plotted against the average D-value at the various temperatures, the temperature curves at or above 50 degrees C were more erratic and the RH had a significant effect. The study showed that temperature and RH must be controlled if biological indicators are to be properly calibrated for use in ethylene oxide sterilization.
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Selected References
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- Kereluk K., Gammon R. A., Lloyd R. S. Microbiological aspects of ethylene oxide sterilization. 3. Effects of humidity and water activity on the sporicidal activity of ethylene oxide. Appl Microbiol. 1970 Jan;19(1):157–162. doi: 10.1128/am.19.1.157-162.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kereluk K., Gammon R. A., Lloyd R. S. Microbiological aspects of ethylene oxide sterilization. II. Microbial resistance to ethylene oxide. Appl Microbiol. 1970 Jan;19(1):152–156. doi: 10.1128/am.19.1.152-156.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kereluk K., Gammon R. A., Lloyd R. S. Microbiological aspects of ethylene oxide sterilization. IV. Influence of thickness of polyethylene film on the sporicidal activity of ethylene oxide. Appl Microbiol. 1970 Jan;19(1):163–165. doi: 10.1128/am.19.1.163-165.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kereluk K., Gammon R. A., Lloyd R. S. Microbiological aspects of ethylene oxide sterization. I. Experimental apparatus and methods. Appl Microbiol. 1970 Jan;19(1):146–151. doi: 10.1128/am.19.1.146-151.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reich R. R., Whitbourne J. E., McDaniel A. W. Effect of storage conditions on the performance of Bacillus stearothermophilus biological indicators. J Parenter Drug Assoc. 1979 Jul-Aug;33(4):228–234. [PubMed] [Google Scholar]