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. 1983 Feb;45(2):622–627. doi: 10.1128/aem.45.2.622-627.1983

Fluorogenic selective and differential medium for isolation of fecal streptococci.

K J Littel, P A Hartman
PMCID: PMC242334  PMID: 6830220

Abstract

Of 44 fluorogenic substrates tested for their ability to differentiate species of fecal streptococci, four yielded species-differentiating reactions. The remaining substrates either yielded uniformly positive, negative, or variable strain-dependent reactions. One substrate, 4-methylumbelliferone-alpha-D-galactoside, was hydrolyzed by Streptococcus bovis and S. faecium and its biotypes. 4-Methylumbelliferone-alpha-D-galactoside and a colorimetric starch substrate were incorporated into the fecal streptococcal selective medium of Donnelly and Hartman (Appl. Environ. Microbiol. 35:576-581, 1978). Three phenotypic groups were identifiable on the new fluorescent gentamicin-thallous-carbonate agar: (i) starch hydrolysis and fluorescence (S. bovis), (ii) no starch hydrolysis but fluorescence (S. faecium and its biotypes), and (iii) no starch hydrolysis or fluorescence (S. faecalis, S. avium, S. equinus, S. mitis, and S. salivarius). Of the presumptive identifications from sewage, swine, and bovine samples, 86% were confirmed as being correct. The new medium has potential application in water, food, environmental, and possibly clinical microbiology.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ceska M. A new approach for quantitative and semi-quantitative determinations of enzymatic activities with simple laboratory equipment. Detection of -amylase. Clin Chim Acta. 1971 Jun;33(1):135–145. doi: 10.1016/0009-8981(71)90260-9. [DOI] [PubMed] [Google Scholar]
  2. DEIBEL R. H. THE GROUP D STREPTOCOCCI. Bacteriol Rev. 1964 Sep;28:330–366. doi: 10.1128/br.28.3.330-366.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Donnelly L. S., Hartman P. A. Gentamicin-based medium for the isolation of group D streptococci and application of the medium to water analysis. Appl Environ Microbiol. 1978 Mar;35(3):576–581. doi: 10.1128/aem.35.3.576-581.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Facklam R. R. Comparison of several laboratory media for presumptive identification of enterococci and group D streptococci. Appl Microbiol. 1973 Aug;26(2):138–145. doi: 10.1128/am.26.2.138-145.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Facklam R. R. Recognition of group D streptococcal species of human origin by biochemical and physiological tests. Appl Microbiol. 1972 Jun;23(6):1131–1139. doi: 10.1128/am.23.6.1131-1139.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Facklam R. R., Thacker L. G., Fox B., Eriquez L. Presumptive identification of streptococci with a new test system. J Clin Microbiol. 1982 Jun;15(6):987–990. doi: 10.1128/jcm.15.6.987-990.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Feng P. C., Hartman P. A. Fluorogenic assays for immediate confirmation of Escherichia coli. Appl Environ Microbiol. 1982 Jun;43(6):1320–1329. doi: 10.1128/aem.43.6.1320-1329.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Geldreich E. E., Kenner B. A. Concepts of fecal streptococci in stream pollution. J Water Pollut Control Fed. 1969 Aug;41(8 Suppl):R336+–R336+. [PubMed] [Google Scholar]
  9. Godsey J. H., Matteo M. R., Shen D., Tolman G., Gohlke J. R. Rapid identification of Enterobacteriaceae with microbial enzyme activity profiles. J Clin Microbiol. 1981 Mar;13(3):483–490. doi: 10.1128/jcm.13.3.483-490.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gross K. C., Houghton M. P., Senterfit L. B. Presumptive speciation of Streptococcus bovis and other group D streptococci from human sources by using arginine and pyruvate tests. J Clin Microbiol. 1975 Jan;1(1):54–60. doi: 10.1128/jcm.1.1.54-60.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hartman P. A., Reinbold G. W., Saraswat D. S. Media and methods for isolation and enumeration of the enterococci. Adv Appl Microbiol. 1966;8:253–289. [PubMed] [Google Scholar]
  12. Klein R. S., Recco R. A., Catalano M. T., Edberg S. C., Casey J. I., Steigbigel N. H. Association of Streptococcus bovis with carcinoma of the colon. N Engl J Med. 1977 Oct 13;297(15):800–802. doi: 10.1056/NEJM197710132971503. [DOI] [PubMed] [Google Scholar]
  13. Lachica R. V., Hartman P. A. Two improved media for isolating and enumerating enterococci in certain frozen foods. J Appl Bacteriol. 1968 Mar;31(1):151–156. doi: 10.1111/j.1365-2672.1968.tb00352.x. [DOI] [PubMed] [Google Scholar]
  14. Oragui J. I., Mara D. D. A selective medium for the enumeration of Streptococcus bovis by membrane filtration. J Appl Bacteriol. 1981 Aug;51(1):85–93. doi: 10.1111/j.1365-2672.1981.tb00911.x. [DOI] [PubMed] [Google Scholar]
  15. Pavlova M. T., Litsky W., Francis F. J. A comparative study of starch hydrolysis by fecal streptococci employing plate and tube techniques. Health Lab Sci. 1971 Apr;8(2):67–74. [PubMed] [Google Scholar]
  16. SWAN A. The use of a bile-aesculin medium and of Maxted's technique of Lancefield grouping in the identification of enterococci (group D streptococci). J Clin Pathol. 1954 May;7(2):160–163. doi: 10.1136/jcp.7.2.160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Thian T. S., Hartman P. A. Gentamicin-thallous-carbonate medium for isolation of fecal streptococci from foods. Appl Environ Microbiol. 1981 Mar;41(3):724–728. doi: 10.1128/aem.41.3.724-728.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wilkowske C. J. Enterococcal endocarditis. Mayo Clin Proc. 1982 Feb;57(2):101–105. [PubMed] [Google Scholar]
  19. Yolken R. H. Enzymic analysis for rapid detection of microbial infection in human body fluids: an overview. Clin Chem. 1981 Sep;27(9):1490–1498. [PubMed] [Google Scholar]

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