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. 1983 Mar;45(3):748–754. doi: 10.1128/aem.45.3.748-754.1983

Eradication of Arizona hinshawii from artificially infected turtle eggs.

S Michael-Marler, M L Brown, R J Siebeling
PMCID: PMC242365  PMID: 6682646

Abstract

Turtle eggs, 24 h old, were infected with Arizona hinshawii and treated 48 h later with gentamicin sulfate (Garasol; Shering Corp., Allantown, N.J.) by pressure differential egg dip treatment to ascertain the concentration of this reagent required to eradicate this pathogen from eggs. Infected eggs treated with 1,000 or 1,500 micrograms of gentamicin per ml of dip solution eliminated detectable A. hinshawii from eggs as determined by testing shells and embryo-yolk homogenates of 12-day-old eggs and the gastrointestinal tracts, kidneys, livers and gall bladders, and yolks of 50-day-old embryos. Treated eggs produced hatchlings which did not excrete detectable A. hinshawii at 72 h or 30 days after hatching, nor was this organism recovered from the visceral organs of these hatchlings when necropsied 30 days after hatching. Bacteriological assays on infected nontreated eggs showed that greater than 70% of the eggs harbored A. hinshawii, and eggs in this group produced hatchlings which actively excreted and harbored A. hinshawii. Eggs not infected or treated also produced turtles which excreted and systemically carried A. hinshawii and Salmonella spp. though not at the same level as did the turtles produced from infected, nontreated eggs.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barnes L. E., Loy J. I., Bickford S. M. Gentamicin levels in chicken eggs and tissues of progeny following temperature-differential dipping. Avian Dis. 1973 Jul-Sep;17(3):594–597. [PubMed] [Google Scholar]
  2. Feeley J. C., Treger M. D. Penetration of turtle eggs by Salmonella braenderup. Public Health Rep. 1969 Feb;84(2):156–158. [PMC free article] [PubMed] [Google Scholar]
  3. Kaufmann A. F., Morrison Z. L. An epidemiologic study of salmonellosis in turtles. Am J Epidemiol. 1966 Sep;84(2):364–370. doi: 10.1093/oxfordjournals.aje.a120649. [DOI] [PubMed] [Google Scholar]
  4. Lucas T. E., Kumar M. C., Kleven S. H., Pomeroy B. S. Antibiotic treatment of turkey hatching eggs preinfected with Salmonellae. Avian Dis. 1970 Aug;14(3):455–462. [PubMed] [Google Scholar]
  5. ROSENSTEIN B. J., RUSSO P., HINCHLIFFE M. C. A FAMILY OUTBREAK OF SALMONELLOSIS TRACED TO A PET TURTLE. N Engl J Med. 1965 May 6;272:960–961. doi: 10.1056/NEJM196505062721807. [DOI] [PubMed] [Google Scholar]
  6. Saif Y. M., Ferguson L. C., Nestor K. E. Treatment of turkey hatching eggs for control of Arizona infection. Avian Dis. 1971 Jul-Sep;15(3):448–461. [PubMed] [Google Scholar]
  7. Saif Y. M., Shelly S. M. Effect of gentamicin sulfate dip on salmonella organisms in experimentally infected turkey eggs. Avian Dis. 1973 Jul-Sep;17(3):574–581. [PubMed] [Google Scholar]
  8. Siebeling R. J., Neal P. M., Granberry W. D. Evaluation of methods for the isolation of Salmonella and Arizona organisms from pet turtles treated with antimicrobial agents. Appl Microbiol. 1975 Feb;29(2):240–245. doi: 10.1128/am.29.2.240-245.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Siebeling R. J., Neal P. M., Granberry W. D. Treatment of Salmonella-Arizona-infected turtle eggs with terramycin and chloromycetin by the temperature-differential egg dip method. Appl Microbiol. 1975 Nov;30(5):791–799. doi: 10.1128/am.30.5.791-799.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Trust T. J., Bartlett K. H. Aquarium pets as a source of antibiotic-resistant salmonellae. Can J Microbiol. 1979 Apr;25(4):535–541. doi: 10.1139/m79-078. [DOI] [PubMed] [Google Scholar]
  11. WILLIAMS L. P., Jr, HELSDON H. L. PET TURTLES AS A CAUSE OF HUMAN SALMONELLOSIS. JAMA. 1965 May 3;192:347–351. doi: 10.1001/jama.1965.03080180005001. [DOI] [PubMed] [Google Scholar]
  12. Wells J. G., Clark G. M., Morris G. K. Evaluation of methods for isolating Salmonella and Arizona organisms from pet turtles. Appl Microbiol. 1974 Jan;27(1):8–10. doi: 10.1128/am.27.1.8-10.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Williams J. E., Dillard L. H., Hall G. O. The penetration patterns of Salmonella typhimurium through the outer structures of chicken eggs. Avian Dis. 1968 Aug;12(3):445–466. [PubMed] [Google Scholar]
  14. Wright G. W., Frank J. F. Penetration of Eggs by Salmonella Typhi-Murium. Can J Comp Med Vet Sci. 1956 Dec;20(12):453–457. [PMC free article] [PubMed] [Google Scholar]

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