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. 1983 Mar;45(3):830–837. doi: 10.1128/aem.45.3.830-837.1983

Effects of Nitrogen Supplements on Degradation of Aspen Wood Lignin and Carbohydrate Components by Phanerochaete chrysosporium†

Ian D Reid 1
PMCID: PMC242379  PMID: 16346246

Abstract

A supplement of KH2PO4, MgSO4, CaCl2, trace elements, and thiamine accelerated the initial rate of aspen wood decay by Phanerochaete chrysosporium but did not increase the extent of lignin degradation. Asparagine, casein hydrolysate, and urea supplements (1% added N) strongly inhibited lignin degradation and weight loss. The complex nitrogen sources peptone and yeast extract stimulated lignin degradation and weight loss. Albumen and NH4Cl had intermediate effects. Conversion of [14C]lignin to 14CO2 and water-soluble materials underestimated lignin degradation in the presence of the complex N sources. The highest ratio of lignin degradation to total weight loss and the largest increase in cellulase digestibility occurred during the decay of unsupplemented wood. Rotting of aspen wood by P. chrysosporium gives smaller digestibility increases than have been found with some other white-rot fungi.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barder M. J., Crawford D. L. Effects of carbon and nitrogen supplementation on lignin and cellulose decomposition by a Streptomyces. Can J Microbiol. 1981 Aug;27(8):859–863. doi: 10.1139/m81-136. [DOI] [PubMed] [Google Scholar]
  2. Crawford D. L., Crawford R. L. Microbial degradation of lignocellulose: the lignin component. Appl Environ Microbiol. 1976 May;31(5):714–717. doi: 10.1128/aem.31.5.714-717.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Eriksson K. E., Hamp S. G. Regulation of Endo-1,4-beta-glucanase production in Sporotrichum pulverulentum. Eur J Biochem. 1978 Sep 15;90(1):183–190. doi: 10.1111/j.1432-1033.1978.tb12589.x. [DOI] [PubMed] [Google Scholar]
  4. Jeffries T. W., Choi S., Kirk T. K. Nutritional Regulation of Lignin Degradation by Phanerochaete chrysosporium. Appl Environ Microbiol. 1981 Aug;42(2):290–296. doi: 10.1128/aem.42.2.290-296.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Keyser P., Kirk T. K., Zeikus J. G. Ligninolytic enzyme system of Phanaerochaete chrysosporium: synthesized in the absence of lignin in response to nitrogen starvation. J Bacteriol. 1978 Sep;135(3):790–797. doi: 10.1128/jb.135.3.790-797.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kirk T. K., Connors W. J., Zeikus J. G. Requirement for a growth substrate during lignin decomposition by two wood-rotting fungi. Appl Environ Microbiol. 1976 Jul;32(1):192–194. doi: 10.1128/aem.32.1.192-194.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Reid I. D. Effects of Nitrogen Sources on Cellulose and Synthetic Lignin Degradation by Phanerochaete chrysosporium. Appl Environ Microbiol. 1983 Mar;45(3):838–842. doi: 10.1128/aem.45.3.838-842.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Reid I. D., Seifert K. A. Lignin degradation by Phanerochaete chrysosporium in hyperbaric oxygen. Can J Microbiol. 1980 Sep;26(9):1168–1171. doi: 10.1139/m80-194. [DOI] [PubMed] [Google Scholar]

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