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. 1983 Mar;45(3):960–965. doi: 10.1128/aem.45.3.960-965.1983

Competition Among Rhizobium leguminosarum Strains for Nodulation of Lentils (Lens esculenta)

Sheila N May 1,†, B Ben Bohlool 1
PMCID: PMC242397  PMID: 16346257

Abstract

Thirty-one cultures of Rhizobium leguminosarum were screened for effectiveness (C2H2 reduction) on lentils (Lens esculenta). Fluorescent antibodies prepared against three of the most effective strains (Hawaii 5-0, Nitragin 92A3, and Nitragin 128A12) exhibited a high degree of strain specificity; the antibodies reacted strongly with their homologous rhizobia in culture and with bacteroids in nodules. They did not cross-react with one another, and only weakly with 5 of the 47 other R. leguminosarum cultures tested. In competition studies in the growth chamber, whenever strain Nitragin 92A3 was included in the inoculum mixture, it consistently (but not always significantly, P = 0.05) occupied the majority of nodules on all four cultivars used. However, some degree of strain X cultivar interaction was apparent: Hawaii 5-0 was of equal competitiveness (P = 0.05) with Nitragin 92A3 on three of the varieties (Commercial, Tekoa, and Benewah), but inferior (P = 0.01) on the Chilean variety; Nitragin 92A3 completely dominated (P = 0.01) Nitragin 128A12 on all cultivars; and Hawaii 5-0 was of equal competitiveness (P = 0.05) to Nitragin 128A12 on the Chilean variety and more competitive (P = 0.01) on the commercial variety and less so on the other two varieties. In field experiments, Hawaii 5-0 proved of equal competitiveness (P = 0.01) with Nitragin 92A3 in one soil (an Inceptisol) and superior (P ≤ 0.05) to it in another (an Oxisol). Incidence of double-strain occupancy of nodules varied from 0 to 36% in vermiculite, depending on the strains in the mixture and the host variety, and from 0 to 38% in the field, depending on the strains in the mixture and the soil type. The results suggest a close relationship between the competitiveness of a strain and its occurrence in doubly infected nodules.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berger J. A., May S. N., Berger L. R., Bohlool B. B. Colorimetric enzyme-linked immunosorbent assay for the identification of strains of Rhizobium in culture and in the nodules of lentils. Appl Environ Microbiol. 1979 Mar;37(3):642–646. doi: 10.1128/aem.37.3.642-646.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bohlool B. B., Schmidt E. L. Nonspecific staining: its control in immunofluorescence examination of soil. Science. 1968 Nov 29;162(3857):1012–1014. doi: 10.1126/science.162.3857.1012. [DOI] [PubMed] [Google Scholar]
  3. Broughton W. J., Dilworth M. J. Control of leghaemoglobin synthesis in snake beans. Biochem J. 1971 Dec;125(4):1075–1080. doi: 10.1042/bj1251075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dorosinskii L. M., Makarova N. M. Konkurentnaia sposobnost' shtammov Rhizobium lupini. Mikrobiologiia. 1977 Jan-Feb;46(1):143–148. [PubMed] [Google Scholar]
  5. Hardy R. W., Holsten R. D., Jackson E. K., Burns R. C. The acetylene-ethylene assay for n(2) fixation: laboratory and field evaluation. Plant Physiol. 1968 Aug;43(8):1185–1207. doi: 10.1104/pp.43.8.1185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Johnston A. W., Beringer J. E. Identification of the rhizobium strains in pea root nodules using genetic markers. J Gen Microbiol. 1975 Apr;87(2):343–350. doi: 10.1099/00221287-87-2-343. [DOI] [PubMed] [Google Scholar]
  7. Leonard L. T. A Simple Assembly for Use in the Testing of Cultures of Rhizobia. J Bacteriol. 1943 Jun;45(6):523–527. doi: 10.1128/jb.45.6.523-527.1943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. READ M. P. The establishment of serologically identifiable strains of Rhizobium trifolii in field soils in competition with the native microflora. J Gen Microbiol. 1953 Aug;9(1):1–14. doi: 10.1099/00221287-9-1-1. [DOI] [PubMed] [Google Scholar]
  9. Schmidt E. L., Bakole R. O., Bohlool B. B. Fluorescent-antibody approach to study of rhizobia in soil. J Bacteriol. 1968 Jun;95(6):1987–1992. doi: 10.1128/jb.95.6.1987-1992.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. VINCENT J. M., WATERS L. M. The influence of the host on competition amongst clover root-nodule bacteria. J Gen Microbiol. 1953 Dec;9(3):357–370. doi: 10.1099/00221287-9-3-357. [DOI] [PubMed] [Google Scholar]

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