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. 1983 Mar;45(3):1140–1143. doi: 10.1128/aem.45.3.1140-1143.1983

Modified method for production and purification of Staphylococcus aureus enterotoxin B.

A K Melconian, J P Flandrois, J Fleurette
PMCID: PMC242421  PMID: 6847182

Abstract

A medium containing 4% bio-trypcase and 1% yeast extract was used for the production of Staphylococcus aureus enterotoxin B. The yield obtained was estimated at 200 micrograms of enterotoxin per ml of S. aureus S-6 culture supernatant. The purification method involves chromatography on Biorex 70 resin, isoelectric focusing, and gel filtration on Sephadex G-100. The purified enterotoxin (isoionic point, pH 8.55) was shown to be homogenous protein with a molecular weight of 29,000 when tested by gel electrophoresis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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