Abstract
Pseudomonas putida cooxidized norcamphor and pericyclocamphanone to hydroxylated and lactonized products during growth on camphor. Norcamphor was hydroxylated at the 5 position, similar to the corresponding process in camphor, but pericyclocamphanone was oxidized at the 6 position. We conclude that the regiochemistry of the hydroxylation may be substrate controlled.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Banerjee S., Dec G. Identification of elimination reactions during the degradation of 5-bromocamphor by P. putida. Biochem Biophys Res Commun. 1980 May 14;94(1):68–70. doi: 10.1016/s0006-291x(80)80188-4. [DOI] [PubMed] [Google Scholar]
- Gould P. V., Gelb M. H., Sligar S. G. Interaction of 5-bromocamphor with cytochrome P-450 cam. Production of 5-ketocamphor from a mixed spin state hemoprotein. J Biol Chem. 1981 Jul 10;256(13):6686–6691. [PubMed] [Google Scholar]
- Groves J. T., McClusky G. A. Aliphatic hydroxylation by highly purified liver microsomal cytochrome P-450. Evidence for a carbon radical intermediate. Biochem Biophys Res Commun. 1978 Mar 15;81(1):154–160. doi: 10.1016/0006-291x(78)91643-1. [DOI] [PubMed] [Google Scholar]
- Gunsalus I. C., Wagner G. C. Bacterial P-450cam methylene monooxygenase components: cytochrome m, putidaredoxin, and putidaredoxin reductase. Methods Enzymol. 1978;52:166–188. doi: 10.1016/s0076-6879(78)52019-3. [DOI] [PubMed] [Google Scholar]
- Trudgill P. W., DuBus R., Gunsalus I. C. Mixed function oxidation. VI. Purification of a tightly coupled electron transport complex in camphor lactonization. J Biol Chem. 1966 Sep 25;241(18):4288–4290. [PubMed] [Google Scholar]