Abstract
To optimize the conversion of carbohydrates to ethanol, strains of several Saccharomyces species were examined for the ability to grow and ferment in a range of sucrose and ethanol concentrations. A total of 632 wine yeasts, most of them isolated from wineries in Andalusia and Extremadura, southwestern Spain, were subjected to screening and selection. Growth and fermentative capacity in different ethanol and sucrose concentrations varied from one strain to another. There was no correlation between growth and fermentative capacity. The best 35 strains grew in 15% ethanol and fermented in 18% ethanol. Ethanol accumulated, although at a reduced rate, after the cells stopped growing. Most yeast strains were highly fermentative in 50% sucrose. Some of them effectively utilized the carbohydrates of the culture, yielding final ethanol concentrations of > 14%. Of the 35 selected strains, 16 were promising for genetic analysis and breeding because of their capacity to sporulate. These strains were homothallic, and their spores were viable. The meiotic products analyzed so far were also homothallic.
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Selected References
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- CHUNG C. W., NICKERSON W. J. Polysaccharide syntheses in growing yeasts. J Biol Chem. 1954 May;208(1):395–407. [PubMed] [Google Scholar]
- DUBOIS M., GILLES K., HAMILTON J. K., REBERS P. A., SMITH F. A colorimetric method for the determination of sugars. Nature. 1951 Jul 28;168(4265):167–167. doi: 10.1038/168167a0. [DOI] [PubMed] [Google Scholar]
- Gray W. D. Further Studies on the Alcohol Tolerance of Yeast: Its Relationship to Cell Storage Products. J Bacteriol. 1948 Jan;55(1):53–59. doi: 10.1128/jb.55.1.53-59.1948. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ismail A. A., Ali A. M. Selection of high ethanol-yielding Saccharomyces. II. Genetics of ethanol tolerance. Folia Microbiol (Praha) 1971;16(5):350–354. doi: 10.1007/BF02875752. [DOI] [PubMed] [Google Scholar]
- Nagodawithana T. W., Steinkraus K. H. Influence of the rate of ethanol production and accumulation on the viability of Saccharomyces cerevisiae in "rapid fermentation". Appl Environ Microbiol. 1976 Feb;31(2):158–162. doi: 10.1128/aem.31.2.158-162.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Navarro J. M., Durand G. Fermentation alcoolique: influence de la température sur l'accumulation d'alcool dans les cellules de levure. Ann Microbiol (Paris) 1978 Aug-Sep;129B(2):215–224. [PubMed] [Google Scholar]
- Navarro J. M. Fermentation alcoolique: influence des conditions de culture sur l'inhibition par l'éthanol. Cell Mol Biol Incl Cyto Enzymol. 1980;26(2):241–246. [PubMed] [Google Scholar]
- Thomas D. S., Hossack J. A., Rose A. H. Plasma-membrane lipid composition and ethanol tolerance in Saccharomyces cerevisiae. Arch Microbiol. 1978 Jun 26;117(3):239–245. doi: 10.1007/BF00738541. [DOI] [PubMed] [Google Scholar]