Abstract
Nitrous oxide production by Escherichia coli seems to result from the reduction of NO2- by NO3- reductase. This hypothesis is consistent with previous observations and with the observation that molybdenum was required for both NO3- reduction and N2O production. Several E. coli NO3- reductase mutants were assayed for both N2O-producing and NO3--reducing activity. The hypothesized role of NO3- reductase is supported by the correlation of these two activities. Nitrate reduction to NH4+ enhanced growth, but NO2- reduction to N2O apparently did not. Therefore, this process differs significantly from respiratory denitrification.
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Selected References
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