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. 1983 Jun;45(6):1775–1779. doi: 10.1128/aem.45.6.1775-1779.1983

Enhanced Mineral Uptake by Zea mays and Sorghum bicolor Roots Inoculated with Azospirillum brasilense†

Willy Lin 1, Yaacov Okon 1,‡, Ralph W F Hardy 1
PMCID: PMC242537  PMID: 16346311

Abstract

Inoculation of corn (Zea mays) seeds with Azospirillum brasilense strain Cd or Sp 7 significantly enhanced (30 to 50% over controls) the uptake of NO3−, K+, and H2PO4− into 3- to 4-day- and 2-week-old root segments. No gross changes in root morphology were observed; altered cell arrangement in the outer four or five layers of the cortex was seen in photomicrographs of cross sections of inoculated corn roots. The surface activity involved in ion uptake probably increased, as shown by the darker staining by methylene blue of the affected area. Shoot dry weight increased 20 to 30% in inoculated plants after 3 weeks, presumably by enhancement of mineral uptake. Corn and sorghum plants grown to maturity on limiting nutrients in the greenhouse showed improved growth from inoculation approaching that of plants grown on normal nutrient concentrations. Enhanced ion uptake may be a significant factor in the crop yield enhancement reported for Azospirillum inoculation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Eskew D. L., Eaglesham A. R., App A. A. Heterotrophic n(2) fixation and distribution of newly fixed nitrogen in a rice-flooded soil system. Plant Physiol. 1981 Jul;68(1):48–52. doi: 10.1104/pp.68.1.48. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Franche C., Elmerich C. Physiological properties and plasmid content of several strains of Azospirillum brasilense and A. lipoferum. Ann Microbiol (Paris) 1981 Jan-Feb;132A(1):3–18. [PubMed] [Google Scholar]
  3. Kapulnik Y., Okon Y., Kigel J., Nur I., Henis Y. Effects of Temperature, Nitrogen Fertilization, and Plant Age on Nitrogen Fixation by Setaria italica Inoculated with Azospirillum brasilense (strain cd). Plant Physiol. 1981 Aug;68(2):340–343. doi: 10.1104/pp.68.2.340. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Lin W. Potassium and Phosphate Uptake in Corn Roots: Further Evidence for an Electrogenic H/K Exchanger and an OH/Pi Antiporter. Plant Physiol. 1979 May;63(5):952–955. doi: 10.1104/pp.63.5.952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Nur I., Okon Y., Henis Y. An increase in nitrogen content of Setaria italica and Zea mays inoculated with Azospirillum. Can J Microbiol. 1980 Apr;26(4):482–485. doi: 10.1139/m80-080. [DOI] [PubMed] [Google Scholar]
  6. Tien T. M., Diem H. G., Gaskins M. H., Hubbell D. H. POlygalacturonic acid transeliminase production by Azospirillum species. Can J Microbiol. 1981 Apr;27(4):426–431. doi: 10.1139/m81-065. [DOI] [PubMed] [Google Scholar]
  7. Tien T. M., Gaskins M. H., Hubbell D. H. Plant Growth Substances Produced by Azospirillum brasilense and Their Effect on the Growth of Pearl Millet (Pennisetum americanum L.). Appl Environ Microbiol. 1979 May;37(5):1016–1024. doi: 10.1128/aem.37.5.1016-1024.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ueda J., Kato J. Isolation and Identification of a Senescence-promoting Substance from Wormwood (Artemisia absinthium L.). Plant Physiol. 1980 Aug;66(2):246–249. doi: 10.1104/pp.66.2.246. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Umali-Garcia M., Hubbell D. H., Gaskins M. H., Dazzo F. B. Association of azospirillum with grass roots. Appl Environ Microbiol. 1980 Jan;39(1):219–226. doi: 10.1128/aem.39.1.219-226.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

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