Abstract
The principle proteolytic bacteria isolated from bovine rumen contents by virtue of the ability to obtain nitrogen from the proteolysis of leaf fraction 1 protein (ribulosebisphosphate carboxylase, EC 4.1.1.39) were identified as Streptococcus bovis and Butyrivibrio spp. Substitution of fresh fodder, rich in soluble protein, for a hay-concentrates diet resulted in enhanced ruminal proteolytic activity and a significant increase in the number of bacteria able to use fraction 1 protein as the sole nitrogen source. Isolated proteolytic bacteria degraded fraction 1 protein and casein readily. Bovine serum albumin was attacked by Butyrivibrio spp. but was resistant to proteolysis by the streptococci.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ABOU AKKADA A. R., BLACKBURN T. H. Some observations on the nitrogen metabolism of rumen proteolytic bacteria. J Gen Microbiol. 1963 Jun;31:461–469. doi: 10.1099/00221287-31-3-461. [DOI] [PubMed] [Google Scholar]
- BLACKBURN T. H., HOBSON P. N. Further studies on the isolation of proteolytic bacteria from the sheep rumen. J Gen Microbiol. 1962 Sep;29:69–81. doi: 10.1099/00221287-29-1-69. [DOI] [PubMed] [Google Scholar]
- Blackburn T. H., Hallah W. A. The cell-bound protease of Bacteroides amylophilus H18. Can J Microbiol. 1974 Apr;20(4):435–441. doi: 10.1139/m74-068. [DOI] [PubMed] [Google Scholar]
- Brock F. M., Forsberg C. W., Buchanan-Smith J. G. Proteolytic activity of rumen microorganisms and effects of proteinase inhibitors. Appl Environ Microbiol. 1982 Sep;44(3):561–569. doi: 10.1128/aem.44.3.561-569.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FULGHUM R. S., MOORE W. E. ISOLATION, ENUMERATION, AND CHARACTERISTICS OF PROTEOLYTIC RUMINAL BACTERIA. J Bacteriol. 1963 Apr;85:808–815. doi: 10.1128/jb.85.4.808-815.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hazlewood G. P., Jones G. A., Mangan J. L. Hydrolysis of leaf Fraction 1 protein by the proteolytic rumen bacterium Bacteroides ruminicola R8/4. J Gen Microbiol. 1981 Apr;123(2):223–232. doi: 10.1099/00221287-123-2-223. [DOI] [PubMed] [Google Scholar]
- Iverson W. G., Millis N. F. Succession of Streptococcus bovis strains with differing bacteriophage sensitivities in the rumens of two fistulated sheep. Appl Environ Microbiol. 1977 Apr;33(4):810–813. doi: 10.1128/aem.33.4.810-813.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kopecny J., Wallace R. J. Cellular location and some properties of proteolytic enzymes of rumen bacteria. Appl Environ Microbiol. 1982 May;43(5):1026–1033. doi: 10.1128/aem.43.5.1026-1033.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nugent J. H., Mangan J. L. Characteristics of the rumen proteolysis of fraction I (18S) leaf protein from lucerne (Medicago sativa L). Br J Nutr. 1981 Jul;46(1):39–58. doi: 10.1079/bjn19810007. [DOI] [PubMed] [Google Scholar]
- Russell J. B., Bottje W. G., Cotta M. A. Degradation of protein by mixed cultures of rumen bacteria: identification of Streptococcus bovis as an actively proteolytic rumen bacterium. J Anim Sci. 1981 Jul;53(1):242–252. doi: 10.2527/jas1981.531242x. [DOI] [PubMed] [Google Scholar]
- Shane B. S., Gouws L., Kistner A. Cellulolytic bacteria occurring in the rumen of sheep conditioned to low-protein teff hay. J Gen Microbiol. 1969 Mar;55(3):445–457. doi: 10.1099/00221287-55-3-445. [DOI] [PubMed] [Google Scholar]
- Siddons R. C., Paradine J. Effect of diet on protein degrading activity in the sheep rumen. J Sci Food Agric. 1981 Oct;32(10):973–981. doi: 10.1002/jsfa.2740321005. [DOI] [PubMed] [Google Scholar]
- TAGARI H., DROR Y., ASCARELLI I., BONDI A. THE INFLUENCE OF LEVELS OF PROTEIN AND STARCH IN RATIONS OF SHEEP ON THE UTILIZATION OF PROTEIN. Br J Nutr. 1964;18:333–356. doi: 10.1079/bjn19640032. [DOI] [PubMed] [Google Scholar]
- Young P. E., Matson M. R., Jones O. W. Amniocentesis for antenatal diagnosis. Review of problems and outcomes in a large series. Am J Obstet Gynecol. 1976 Jun 15;125(4):495–501. [PubMed] [Google Scholar]