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. 1983 Jun;45(6):1838–1847. doi: 10.1128/aem.45.6.1838-1847.1983

Improved conversion of fumarate to succinate by Escherichia coli strains amplified for fumarate reductase.

I Goldberg, K Lonberg-Holm, E A Bagley, B Stieglitz
PMCID: PMC242547  PMID: 6349526

Abstract

Two recombinant plasmid Escherichia coli strains containing amplified fumarate reductase activity converted fumarate to succinate at significantly higher rates and yields than a wild-type E. coli strain. Glucose was required for the conversion of fumarate to succinate, and in the absence of glucose or in cultures with a low cell density, malate accumulated. Two-dimensional gel electrophoretic analysis of proteins from the recombinant DNA and wild-type strains showed that increased quantities of both large and small fumarate reductase subunits were expressed in the recombinant DNA strains.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cole S. T., Guest J. R. Production of a soluble form of fumarate reductase by multiple gene duplication in Escherichia coli K12. Eur J Biochem. 1979 Dec;102(1):65–71. doi: 10.1111/j.1432-1033.1979.tb06263.x. [DOI] [PubMed] [Google Scholar]
  2. Dickie P., Weiner J. H. Purification and characterization of membrane-bound fumarate reductase from anaerobically grown Escherichia coli. Can J Biochem. 1979 Jun;57(6):813–821. doi: 10.1139/o79-101. [DOI] [PubMed] [Google Scholar]
  3. Guest J. R. Partial replacement of succinate dehydrogenase function by phage- and plasmid-specified fumarate reductase in Escherichia coli. J Gen Microbiol. 1981 Feb;122(2):171–179. doi: 10.1099/00221287-122-2-171. [DOI] [PubMed] [Google Scholar]
  4. Kahn M., Kolter R., Thomas C., Figurski D., Meyer R., Remaut E., Helinski D. R. Plasmid cloning vehicles derived from plasmids ColE1, F, R6K, and RK2. Methods Enzymol. 1979;68:268–280. doi: 10.1016/0076-6879(79)68019-9. [DOI] [PubMed] [Google Scholar]
  5. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  6. Lohmeier E., Hagen D. S., Dickie P., Weiner J. H. Cloning and expression of fumarate reductase gene of Escherichia coli. Can J Biochem. 1981 Mar;59(3):158–164. doi: 10.1139/o81-023. [DOI] [PubMed] [Google Scholar]
  7. Lonberg-Holm K., Bagley E. A., Nusbacher J., Heal J. M. Two-dimensional gel electrophoresis method for investigation of human plasma proteins: detection of subtle changes during filtration leukapheresis. Clin Chem. 1982 Apr;28(4 Pt 2):962–968. [PubMed] [Google Scholar]
  8. O'Farrell P. Z., Goodman H. M., O'Farrell P. H. High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell. 1977 Dec;12(4):1133–1141. doi: 10.1016/0092-8674(77)90176-3. [DOI] [PubMed] [Google Scholar]
  9. Spencer M. E., Guest J. R. Isolation and properties of fumarate reductase mutants of Escherichia coli. J Bacteriol. 1973 May;114(2):563–570. doi: 10.1128/jb.114.2.563-570.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

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