Abstract
Protoplast formation, fusion, and cell regeneration have been achieved with mutant strains of Actinoplanes brasiliensis. Three-, four-, and five-factor crosses have shown genetic recombination among the markers, and a five-factor cross is analyzed and discussed. Possibilities of using protoplast fusion for gene mapping and strain improvement are suggested.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baltz R. H. Genetic recombination in Streptomyces fradiae by protoplast fusion and cell regeneration. J Gen Microbiol. 1978 Jul;107(1):93–102. doi: 10.1099/00221287-107-1-93. [DOI] [PubMed] [Google Scholar]
- Hopwood D. A., Wright H. M. Bacterial protoplast fusion: recombination in fused protoplasts of Streptomyces coelicolor. Mol Gen Genet. 1978 Jul 4;162(3):307–317. doi: 10.1007/BF00268856. [DOI] [PubMed] [Google Scholar]
- Hopwood D. A., Wright H. M., Bibb M. J., Cohen S. N. Genetic recombination through protoplast fusion in Streptomyces. Nature. 1977 Jul 14;268(5616):171–174. doi: 10.1038/268171a0. [DOI] [PubMed] [Google Scholar]
- Okanishi M., Suzuki K., Umezawa H. Formation and reversion of Streptomycete protoplasts: cultural condition and morphological study. J Gen Microbiol. 1974 Feb;80(2):389–400. doi: 10.1099/00221287-80-2-389. [DOI] [PubMed] [Google Scholar]
- Parenti F., Coronelli C. Members of the genus Actinoplanes and their antibiotics. Annu Rev Microbiol. 1979;33:389–411. doi: 10.1146/annurev.mi.33.100179.002133. [DOI] [PubMed] [Google Scholar]