Abstract
Growth of Zymomonas mobilis ATCC 29191 and CP4 in a continuous stirred tank fermentor resulted in the selection of stable flocculating variants. Factors responsible for enhancing the system pressures selective for the morphological variants were identified. By incorporating some modifications into the design of the fermentor, it was possible to achieve steady-state operation of the chemostat with both wild-type and flocculating strains. Biochemical and microscopic studies were performed to elucidate the mechanism of flocculation in Z. mobilis.
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Costerton J. W., Geesey G. G., Cheng K. J. How bacteria stick. Sci Am. 1978 Jan;238(1):86–95. doi: 10.1038/scientificamerican0178-86. [DOI] [PubMed] [Google Scholar]
- Deinema M. H., Zevenhuizen L. P. Formation of cellulose fibrils by gram-negative bacteria and their role in bacterial flocculation. Arch Mikrobiol. 1971;78(1):42–51. doi: 10.1007/BF00409087. [DOI] [PubMed] [Google Scholar]
- Eveleigh D. E., Montenecourt B. S. Increasing yields of extracellular enzymes. Adv Appl Microbiol. 1979;25:57–74. doi: 10.1016/s0065-2164(08)70146-1. [DOI] [PubMed] [Google Scholar]
- Harder W., Kuenen J. G. A review. Microbial selection in continuous culture. J Appl Bacteriol. 1977 Aug;43(1):1–24. doi: 10.1111/j.1365-2672.1977.tb00717.x. [DOI] [PubMed] [Google Scholar]
- Jones I. M., Primrose S. B., Robinson A., Ellwood D. C. Maintenance of some ColE1-type plasmids in chemostat culture. Mol Gen Genet. 1980;180(3):579–584. doi: 10.1007/BF00268063. [DOI] [PubMed] [Google Scholar]
- Kanas N., Rogers M., Kreth E., Patterson L., Campbell R. The effectiveness of group psychotherapy during the first three weeks of hospitalization. A controlled study. J Nerv Ment Dis. 1980 Aug;168(8):487–492. doi: 10.1097/00005053-198008000-00007. [DOI] [PubMed] [Google Scholar]
- NOVICK A., SZILARD L. Experiments with the Chemostat on spontaneous mutations of bacteria. Proc Natl Acad Sci U S A. 1950 Dec;36(12):708–719. doi: 10.1073/pnas.36.12.708. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schlegel H. G., Jannasch H. W. Enrichment cultures. Annu Rev Microbiol. 1967;21:49–70. doi: 10.1146/annurev.mi.21.100167.000405. [DOI] [PubMed] [Google Scholar]
- Swings J., De Ley J. The biology of Zymomonas. Bacteriol Rev. 1977 Mar;41(1):1–46. doi: 10.1128/br.41.1.1-46.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yada R. Y., Skura B. J. Scanning electron microscope study of Pseudomonas fragi on intact and sarcoplasm-depleted bovine longissimus dorsi muscle. Appl Environ Microbiol. 1982 Apr;43(4):905–915. doi: 10.1128/aem.43.4.905-915.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]