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. 1983 Jun;45(6):1949–1952. doi: 10.1128/aem.45.6.1949-1952.1983

Rapid assay for detection of microorganisms producing DNA-damaging metabolites.

G Mazza
PMCID: PMC242566  PMID: 6410992

Abstract

Microorganisms producing DNA-damaging metabolites (i.e., fungi and streptomycetes) were detected by the Bacillus subtilis rec assay with agar plugs from plates on which the microorganisms had been grown. This assay allowed rapid identification of aflatoxinogenic fungi and streptomycetes producing strong DNA-damaging metabolites. For screening programs, several media have to be used to grow the microorganisms to be tested.

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Selected References

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  1. Benedict W. F., Baker M. S., Haroun L., Choi E., Ames B. N. Mutagenicity of cancer chemotherapeutic agents in the Salmonella/microsome test. Cancer Res. 1977 Jul;37(7 Pt 1):2209–2213. [PubMed] [Google Scholar]
  2. Butler W. H., Barnes J. M. Carcinogenic action of groundnut meal containing aflatoxin in rats. Food Cosmet Toxicol. 1968 Aug;6(2):135–141. doi: 10.1016/0015-6264(68)90194-6. [DOI] [PubMed] [Google Scholar]
  3. Davis N. D., Diener U. L., Eldridge D. W. Production of aflatoxins B1 and G1 by Aspergillus flavus in a semisynthetic medium. Appl Microbiol. 1966 May;14(3):378–380. doi: 10.1128/am.14.3.378-380.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Enomoto M., Saito M. Carcinogens produced by fungi. Annu Rev Microbiol. 1972;26:279–312. doi: 10.1146/annurev.mi.26.100172.001431. [DOI] [PubMed] [Google Scholar]
  5. Garner R. C., Miller E. C., Miller J. A. Liver microsomal metabolism of aflatoxin B 1 to a reactive derivative toxic to Salmonella typhimurium TA 1530. Cancer Res. 1972 Oct;32(10):2058–2066. [PubMed] [Google Scholar]
  6. Kada T., Tutikawa K., Sadaie Y. In vitro and host-mediated "rec-assay" procedures for screening chemical mutagens; and phloxine, a mutagenic red dye detected. Mutat Res. 1972 Oct;16(2):165–174. doi: 10.1016/0027-5107(72)90177-7. [DOI] [PubMed] [Google Scholar]
  7. Kuczuk M. H., Benson P. M., Heath H., Hayes A. W. Evaluation of the mutagenic potential of mycotoxins using Salmonella typhimurium and Saccharomyces cerevisiae. Mutat Res. 1978 Feb;53(1):11–20. doi: 10.1016/0165-1161(78)90375-8. [DOI] [PubMed] [Google Scholar]
  8. Leifer Z., Kada T., Mandel M., Zeiger E., Stafford R., Rosenkranz H. S. An evaluation of tests using DNA repair-deficient bacteria for predicting genotoxicity and carcinogenicity. A report of the U.S. EPA's Gene-TOX Program. Mutat Res. 1981 Nov;87(3):211–297. doi: 10.1016/0165-1110(81)90015-4. [DOI] [PubMed] [Google Scholar]
  9. Mazza G. Bacillus subtilis "rec assay" test with isogenic strains. Appl Environ Microbiol. 1982 Jan;43(1):177–184. doi: 10.1128/aem.43.1.177-184.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McCann J., Ames B. N. Detection of carcinogens as mutagens in the Salmonella/microsome test: assay of 300 chemicals: discussion. Proc Natl Acad Sci U S A. 1976 Mar;73(3):950–954. doi: 10.1073/pnas.73.3.950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McCann J., Choi E., Yamasaki E., Ames B. N. Detection of carcinogens as mutagens in the Salmonella/microsome test: assay of 300 chemicals. Proc Natl Acad Sci U S A. 1975 Dec;72(12):5135–5139. doi: 10.1073/pnas.72.12.5135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Miller J. A., Miller E. C. Carcinogens occurring naturally in foods. Fed Proc. 1976 May 1;35(6):1316–1321. [PubMed] [Google Scholar]
  13. Ong T. M. Aflatoxin mutagenesis. Mutat Res. 1975;32(1):35–53. doi: 10.1016/0165-1110(75)90010-x. [DOI] [PubMed] [Google Scholar]
  14. Otsuji N., Horiuchi T., Nakata A., Kawamata J. Strains of Escherichia coli hypersensitive to representative carcinostatic and carcinogenic agents. J Antibiot (Tokyo) 1978 Aug;31(8):794–796. doi: 10.7164/antibiotics.31.794. [DOI] [PubMed] [Google Scholar]
  15. Raina V., Gurtoo H. L., Berrigan M., Paigen B. Genetic dependence of hepatic microsome-mediated depression of aflatoxin B1 activation to mutagens in Ames Salmonella typhimurium TA-98 system. Biochem Biophys Res Commun. 1980 Jun 16;94(3):737–743. doi: 10.1016/0006-291x(80)91297-8. [DOI] [PubMed] [Google Scholar]
  16. Rinkus S. J., Legator M. S. Chemical characterization of 465 known or suspected carcinogens and their correlation with mutagenic activity in the Salmonella typhimurium system. Cancer Res. 1979 Sep;39(9):3289–3318. [PubMed] [Google Scholar]
  17. Stark A. A. Mutagenicity and carcinogenicity of mycotoxins: DNA binding as a possible mode of action. Annu Rev Microbiol. 1980;34:235–262. doi: 10.1146/annurev.mi.34.100180.001315. [DOI] [PubMed] [Google Scholar]
  18. Suter W., Jaeger I. Comparative evaluation of different pairs of DNA repair-deficient and DNA repair-proficient bacterial tester strains for rapid detection of chemical mutagens and carcinogens. Mutat Res. 1982 Feb;97(1):1–18. doi: 10.1016/0165-1161(82)90015-2. [DOI] [PubMed] [Google Scholar]
  19. Ueno Y., Kubota K. DNA-attacking ability of carcinogenic mycotoxins in recombination-deficient mutant cells of Bacillus subtilis. Cancer Res. 1976 Feb;36(2 Pt 1):445–451. [PubMed] [Google Scholar]
  20. Wehner F. C., Thiel P. G., van Rensburg S. J., Demasius I. P. Mutagenicity to Salmonella typhimurium of some Aspergillus and Penicillium mycotoxins. Mutat Res. 1978 Nov;58(2-3):193–203. doi: 10.1016/0165-1218(78)90009-5. [DOI] [PubMed] [Google Scholar]
  21. Wogan G. N., Newberne P. M. Dose-response characteristics of aflatoxin B1 carcinogenesis in the rat. Cancer Res. 1967 Dec;27(12):2370–2376. [PubMed] [Google Scholar]
  22. Yoshimoto A., Oki T., Inui T. Differential antimicrobial activities of anthracycline antibiotics on rec-Bacillus subtilis. J Antibiot (Tokyo) 1978 Jan;31(1):92–94. doi: 10.7164/antibiotics.31.92. [DOI] [PubMed] [Google Scholar]

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