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. 1977 Aug;34(2):115–119. doi: 10.1128/aem.34.2.115-119.1977

Effect of Tween 80 on glucosyltransferase production in Streptococcus mutans.

Y Umesaki, Y Kawai, M Mutai
PMCID: PMC242607  PMID: 907338

Abstract

Glucan production from sucrose by Streptococcus mutans OMZ 176 was stimulated approximately threefold in the presence of 0.1% Tween 80. When OMZ 176 was grown in a medium containing glucose, the glucosyltransferase level in the medium was also increased about fivefold in the presence of 0.1% Tween 80. The glucosyltransferase level increased in proportion to the logarithm of the concentration of Tween 80 in the glucose medium. Tween 80 affected neither bacterial growth nor the activity of glucosyltransferase. The appearance of glucosyltransferase in the glucose medium was inhibited immediately by chloramphenicol and actinomycin D and, after a lag, by rifampin as well. It was observed that the fatty acid composition of the cells grown with Tween 80 was altered. These results suggest that Tween 80 stimulates glucosyltransferase synthesis either directly, or indirectly by promoting glucosyltransferase secretion.

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Selected References

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  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. EFTHYMIOU C., HANSEN P. A. An antigenic analysis of Lactobacillus acidophilus. J Infect Dis. 1962 May-Jun;110:258–267. doi: 10.1093/infdis/110.3.258. [DOI] [PubMed] [Google Scholar]
  3. Fukui K., Fukui Y., Moriyama T. Purification and properties of dextransucrase and invertase from Streptococcus mutans. J Bacteriol. 1974 Jun;118(3):796–804. doi: 10.1128/jb.118.3.796-804.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Germaine G. R., Chludzinski A. M., Schachtele C. F. Streptococcus mutans dextransucrase: requirement for primer dextran. J Bacteriol. 1974 Oct;120(1):287–294. doi: 10.1128/jb.120.1.287-294.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gibbons R. J., Banghart S. B. Synthesis of extracellular dextran by cariogenic bacteria and its presence in human dental plaque. Arch Oral Biol. 1967 Jan;12(1):11–23. doi: 10.1016/0003-9969(67)90137-9. [DOI] [PubMed] [Google Scholar]
  6. Gibbons R. J., Fitzgerald R. J. Dextran-induced agglutination of Streptococcus mutans, and its potential role in the formation of microbial dental plaques. J Bacteriol. 1969 May;98(2):341–346. doi: 10.1128/jb.98.2.341-346.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Guggenheim B., Newbrun E. Extracellular glucosyltransferase activity of an HS strain of Streptococcus mutans. Helv Odontol Acta. 1969 Oct;13(2):84–97. [PubMed] [Google Scholar]
  8. Jablonski W. M., Hayashi J. A. Inhibition of extracellular streptococcal enzymes. J Dent Res. 1970 Jan-Feb;49(1):178–178. doi: 10.1177/00220345700490011801. [DOI] [PubMed] [Google Scholar]
  9. Kuramitsu H. K. Characterization of cell-associated dextransucrase activity from glucose-grown cells of Streptococcus mutans. Infect Immun. 1974 Jul;10(1):227–235. doi: 10.1128/iai.10.1.227-235.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McCabe M. M., Smith E. E. Origin of the cell-associated dextransucrase of Streptococcus mutans. Infect Immun. 1973 Jun;7(6):829–838. doi: 10.1128/iai.7.6.829-838.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Perch B., Kjems E., Ravn T. Biochemical and serological properties of Streptococcus mutans from various human and animal sources. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Jun;82(3):357–370. doi: 10.1111/j.1699-0463.1974.tb02338.x. [DOI] [PubMed] [Google Scholar]
  12. Reese E. T., Maguire A. Surfactants as stimulants of enzyme production by microorganisms. Appl Microbiol. 1969 Feb;17(2):242–245. doi: 10.1128/am.17.2.242-245.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Robrish S. A., Reid W., Krichevsky M. I. Distribution of enzymes forming polysaccharide from sucrose and the composition of extracellular polysaccharide synthesized by Streptococcus mutans. Appl Microbiol. 1972 Aug;24(2):184–190. doi: 10.1128/am.24.2.184-190.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Scales W. R., Long L. W., Edwards J. R. Purification and characterization of a glycosyltransferase complex from the culture broth of Streptococcus mutans FA1. Carbohydr Res. 1975 Jul;42(2):325–338. doi: 10.1016/s0008-6215(00)84274-3. [DOI] [PubMed] [Google Scholar]
  15. Turesky S., Glickman I., Sandberg R. In vitro chemical inhibition of plaque formation. J Periodontol. 1972 May;43(5):263–269. doi: 10.1902/jop.1972.43.5.263. [DOI] [PubMed] [Google Scholar]
  16. WELKER N. E., CAMPBELL L. L. DE NOVO SYNTHESIS OF ALPHA-AMYLASE BY BACILLUS STEAROTHERMOPHILUS. J Bacteriol. 1963 Dec;86:1202–1210. doi: 10.1128/jb.86.6.1202-1210.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]

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