Skip to main content
NCBI home page
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1977 Sep;34(3):270–273. doi: 10.1128/aem.34.3.270-273.1977

Increased recovery of salmonellae from environmental samples enriched with buffered peptone water.

B M Thomason, D J Dodd, W B Cherry
PMCID: PMC242641  PMID: 334073

Abstract

The incidence and persistence of salmonellae in weather pools on the top of Stone Mountain were investigated with lactose and buffered peptone water used as pre-enrichment broths. A total of 162 samples were collected from 16 weather pools over a 3-month period. The use of buffered peptone water increased the recovery of salmonellae by approximately 25%. The combined use of direct enrichment in tetrathionate broth containing brilliant green dye and pre-enrichment in buffered peptone water followed by enrichment in tetrathionate broth made it possible to detect all 37 of the contaminated samples. All of the isolates were Salmonella bareilly, the only serotype recovered in a previous study. All but one of the isolations were made from moist or wet samples. S. bareilly was isolated from rabbit dung and litter collected near the weather pools, but attempts to trap rabbits for study were unsuccessful. Random samples taken along a side of the mountain yielded S. bareilly in weather pools within the upper third of the mountain; below this level, S. weslaco and S. memphis were recovered, but not S. bareilly.

Full text

PDF
270

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLEN L. A., PASLEY S. M., PIERCE M. S. F. Conditions affecting the growth of Bacterium coli on bile salts media; enumeration of this organism in polluted waters. J Gen Microbiol. 1952 Nov;7(3-4):257–267. doi: 10.1099/00221287-7-3-4-257. [DOI] [PubMed] [Google Scholar]
  2. Alford J. A., Knight N. L. Applicability of aeration and delayed addition of selenite to the isolation of salmonellae. Appl Microbiol. 1969 Dec;18(6):1060–1064. doi: 10.1128/am.18.6.1060-1064.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bissonnette G. K., Jezeski J. J., McFeters G. A., Stuart D. G. Influence of environmental stress on enumeration of indicator bacteria from natural waters. Appl Microbiol. 1975 Feb;29(2):186–194. doi: 10.1128/am.29.2.186-194.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Carlson V. L., Snoeyenbos G. H. Comparative efficacies of selenite and tetrathionate enrichment broths for the isolation of Salmonella serotypes. Am J Vet Res. 1974 May;35(5):711–718. [PubMed] [Google Scholar]
  5. Cherry W. B., Hanks J. B., Thomason B. M., Murlin A. M., Biddle J. W., Croom J. M. Salmonellae as an index of pollution of surface waters. Appl Microbiol. 1972 Sep;24(3):334–340. doi: 10.1128/am.24.3.334-340.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cherry W. B., Thomason B. M., Gladden J. B., Holsing N., Murlin A. M. Detection of salmonellae in foodstuffs, feces, and water by immunofluorescence. Ann N Y Acad Sci. 1975 Jun 30;254:350–368. doi: 10.1111/j.1749-6632.1975.tb29186.x. [DOI] [PubMed] [Google Scholar]
  7. Edel W., Kampelmacher E. H. Comparative studies on the isolation of "sublethally injured" salmonellae in nine European laboratories. Bull World Health Organ. 1973;48(2):167–174. [PMC free article] [PubMed] [Google Scholar]
  8. Kaper J. B., Sayler G. S., Baldini M. M., Colwell R. R. Ambient-temperature primary nonselective enrichment for isolation of Salmonella spp. from an estuarine environment. Appl Environ Microbiol. 1977 Apr;33(4):829–835. doi: 10.1128/aem.33.4.829-835.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mossel D. A., Ratto M. A. Rapid detection of sublethally impaired cells of Enterobacteriaceae in dried foods. Appl Microbiol. 1970 Aug;20(2):273–275. doi: 10.1128/am.20.2.273-275.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. NORTH W. R., Jr Lactose pre-enrichment method for isolation of Samonella from dried egg albumen. Its use in a survery of commercially produced albumen. Appl Microbiol. 1961 May;9:188–195. doi: 10.1128/am.9.3.188-195.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Thomason B. M., Biddle J. W., Cherry W. B. Dection of salmonellae in the environment. Appl Microbiol. 1975 Nov;30(5):764–767. doi: 10.1128/am.30.5.764-767.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Thomason B. M., Dodd D. J. Comparison of enrichment procedures for fluorescent antibody and cultural detection of salmonellae in raw meat and poultry. Appl Environ Microbiol. 1976 May;31(5):787–788. doi: 10.1128/aem.31.5.787-788.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Thomason B. M., Wells J. G. Preparation and testing of polyvalent conjugates for fluorescent-antibody detection of salmonellae. Appl Microbiol. 1971 Nov;22(5):876–884. doi: 10.1128/am.22.5.876-884.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES