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. 1977 Oct;34(4):371–376. doi: 10.1128/aem.34.4.371-376.1977

Nutritional and biochemical characterization of Methanospirillum hungatii.

J G Ferry, R S Wolfe
PMCID: PMC242665  PMID: 411420

Abstract

To ascertain its physiological similarity to other methanogenic bacteria, Methanospirillum hungatii, the type species of the genus, was characterized nutritionally and biochemically. Good growth occurred in a medium consisting of mineral salts, cysteine sulfide reducing buffer, and an H2-CO2 (80:20) atmosphere. Addition of amino acids and B vitamins stimulated growth. Cell-free extracts contained methylcobalamin-coenzyme M methyltransferase, methylreductase, and formate hydrogenlyase. Cells contained coenzyme M and coenzyme F420. Coenzyme F420 was required for formate hydrogenlyase activity. Coenzyme F420 purified from M. hungatii had identical properties to that purified from species of Methanobacterium. The physiological basis of the family Methanobacteriaceae is strengthened by these findings.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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