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. 1977 Nov;34(5):465–472. doi: 10.1128/aem.34.5.465-472.1977

L-Histidine production by histidase-less regulatory mutants of Serratia marcescens constructed by transduction.

M Kisumi, N Nakanishi, T Takagi, I Chibata
PMCID: PMC242684  PMID: 337893

Abstract

2-Methylhistidine (2MH) and 1,2,4-triazole-3-alanine (TRA) inhibited the growth of Serratia marcescens. These inhibitory effects were counteracted by L-histidine. Enzymatic studies showed that 2MH acts as a false feedback inhibitor and TRA acts as both a false feedback inhibitor and a repressor. Mutants resistant to each analog were isolated from a histidase-less mutant, because the wild-type strain possesses a potent histidase activity. 2MH-resistant mutants had a feedback-insensitive phosphoribosyltransferase, but they produced only small amounts of L-histidine. TRA-resistant mutants were divided into two types according to their histidine productivity. A mutant of one type produced about 8 mg of L-histidine per ml and had about a 10-fold increase in the enzyme levels of histidine biosynthesis. Moreover, this mutant had a partially feedback-insensitive phosphoribosyltransferase. A mutant of the second type produced only a small amount of L-histidine and had only derepressed enzyme levels. Accordingly, strains possessing the genetic alterations in both 2MH- and TRA-resistant mutants were constructed by PS20-mediated transduction. They had both feedback-insensitive phosphoribosyltransferase and derepressed enzyme levels. The representative strain HT-2604 produced about 17 mg of L-histidine per ml.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BELSER W. L., BUNTING M. I. Studies on a mechanism providing for genetic transfer in Serratia marcescens. J Bacteriol. 1956 Nov;72(5):582–592. doi: 10.1128/jb.72.5.582-592.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brenner M., Ames B. N. Histidine regulation in Salmonella typhimurium. IX. Histidine transfer ribonucleic acid of the regulatory mutants. J Biol Chem. 1972 Feb 25;247(4):1080–1088. [PubMed] [Google Scholar]
  3. Brill W. J., Magasanik B. Genetic and metabolic control of histidase and urocanase in Salmonella typhimurium, strain 15-59. J Biol Chem. 1969 Oct 10;244(19):5392–5402. [PubMed] [Google Scholar]
  4. Calhoun D. H., Jensen R. A. Significance of altered carbon flow in aromatic amino acid synthesis: an approach to the isolation of regulatory mutants in Pseudomonas aeruginosa. J Bacteriol. 1972 Jan;109(1):365–372. doi: 10.1128/jb.109.1.365-372.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hilton J. L., Kearney P. C., Ames B. N. Mode of action of the herbicide, 3-amino-1,2,4-triazole(amitrole): inhibition of an enzyme of histidine biosynthesis. Arch Biochem Biophys. 1965 Dec;112(3):544–547. doi: 10.1016/0003-9861(65)90093-7. [DOI] [PubMed] [Google Scholar]
  7. Kisumi M., Kato J., Komatsubara S., Chibata I. Increase in isoleucine accumulation by alpha-aminobutyric acid-resistant mutants of Serratia marcescens. Appl Microbiol. 1971 Apr;21(4):569–574. doi: 10.1128/am.21.4.569-574.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kisumi M., Komatsubara S., Chibata I. Leucine accumulation by isoleucine revertants of Serratia marcescens resistant to -aminobutyric acid: lack of both feedback inhibition and repression. J Biochem. 1973 Jan;73(1):107–115. [PubMed] [Google Scholar]
  9. Kisumi M., Komatsubara S., Chibata I. Valine accumulation by alpha-aminobutyric acid-resistant mutants of Serratia marcescens. J Bacteriol. 1971 May;106(2):493–499. doi: 10.1128/jb.106.2.493-499.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kisumi M., Komatsubara S., Sugiura M., Chibata I. Isoleucine accumulation by regulatory mutants of Serratia marcescens: lack of both feedback inhibition and repression. J Bacteriol. 1972 May;110(2):761–763. doi: 10.1128/jb.110.2.761-763.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kisumi M., Komatsubara S., Sugiura M., Chibata I. Properties of isoleucine hydroxamate-resistant mutants of Serratia marcescens. J Gen Microbiol. 1971 Dec;69(3):291–297. doi: 10.1099/00221287-69-3-291. [DOI] [PubMed] [Google Scholar]
  12. Kisumi M., Sugiura M., Takagi T., Chibata I. Norvaline accumulation by regulatory mutants of Serratia marcescens. J Antibiot (Tokyo) 1977 Jan;30(1):111–117. doi: 10.7164/antibiotics.30.111. [DOI] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Matsumoto H., Tazaki T., Hosogaya S. A generalized transducing phage of Serratia marcescens. Jpn J Microbiol. 1973 Nov;17(6):473–479. doi: 10.1111/j.1348-0421.1973.tb00933.x. [DOI] [PubMed] [Google Scholar]
  15. Matsumoto H., Tazaki T., Hosogaya S. Transductional analysis of the leu-thr region of chromosome of Serratia marcescens. J Bacteriol. 1974 Mar;117(3):1365–1367. doi: 10.1128/jb.117.3.1365-1367.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Meiss H. K., Brill W. J., Magasanik B. Genetic control of histidine degradation in Salmonella typhimurium, strain LT-2. J Biol Chem. 1969 Oct 10;244(19):5382–5391. [PubMed] [Google Scholar]
  17. ONO T., HIROHATA R. Synthese des Ophidins. Hoppe Seylers Z Physiol Chem. 1956 May 28;304(2-4):77–81. [PubMed] [Google Scholar]
  18. Roth J. R., Antón D. N., Hartman P. E. Histidine regulatory mutants in Salmonella typhimurium. I. Isolation and general properties. J Mol Biol. 1966 Dec 28;22(2):305–323. doi: 10.1016/0022-2836(66)90134-3. [DOI] [PubMed] [Google Scholar]
  19. SHEPPARD D. E. MUTANTS OF SALMONELLA TYPHIMURIUM RESISTANT TO FEEDBACK INHIBITION BY L-HISTIDINE. Genetics. 1964 Oct;50:611–623. doi: 10.1093/genetics/50.4.611. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. SMOGYI M. Notes on sugar determination. J Biol Chem. 1952 Mar;195(1):19–23. [PubMed] [Google Scholar]
  21. Steiger H., Müller U., Bauer G. Non-receptivity for kappa phage of kappa-lysogenic Serratia and reactions to superinfection of receptive cells with a mutant prophage. Mol Gen Genet. 1972;114(4):358–367. doi: 10.1007/BF00267504. [DOI] [PubMed] [Google Scholar]

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