Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1977 Nov;34(5):473–477. doi: 10.1128/aem.34.5.473-477.1977

Rapid identification and quantitation of small numbers of microorganisms by a chemiluminescent immunoreaction.

M Halmann, B Velan, T Sery
PMCID: PMC242685  PMID: 337894

Abstract

A method (patent pending) for rapidly identifying and quantitating small numbers of microorganisms was developed based on the specific immunoreaction of microorganisms with homologous antibodies linked by conjugation to peroxidase. The high sensitivity of the method is due to the use of a chemiluminescent reaction for the determination of the enzyme. The reaction was performed on Alcar supports with low nonspecific adsorption. The very low noise achieved permitted the detection of as few as 30 to 300 bacterial cells.

Full text

PDF
473

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avrameas S., Ternynck T. Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration. Immunochemistry. 1971 Dec;8(12):1175–1179. doi: 10.1016/0019-2791(71)90395-8. [DOI] [PubMed] [Google Scholar]
  2. Benbough J. E., Martin K. L. An indirect radiolabelled antibody staining technique for the rapid detection and identification of bacteria. J Appl Bacteriol. 1976 Aug;41(1):47–58. doi: 10.1111/j.1365-2672.1976.tb00604.x. [DOI] [PubMed] [Google Scholar]
  3. Frackelton A. R., Jr, Szaro R. P., Weltman J. K. A galactosidase immunosorbent test for carcinoembryonic antigen. Cancer Res. 1976 Aug;36(8):2845–2849. [PubMed] [Google Scholar]
  4. Saunders G. C., Clinard E. H. Rapid micromethod of screening for antibodies to disease agents using the indirect enzyme-labeled antibody test. J Clin Microbiol. 1976 Jun;3(6):604–608. doi: 10.1128/jcm.3.6.604-608.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Sternberger L. A., Hardy P. H., Jr, Cuculis J. J., Meyer H. G. The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem. 1970 May;18(5):315–333. doi: 10.1177/18.5.315. [DOI] [PubMed] [Google Scholar]
  6. Strange R. E., Martin K. L. Rapid assays for the detection and determination of sparse populations of bacteria and bacteriophage T7 with radioactively labelled homologous antibodies. J Gen Microbiol. 1972 Aug;72(1):127–141. doi: 10.1099/00221287-72-1-127. [DOI] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES