Abstract
A simple double-antibody enzyme immunoassay that uses a microtechnique was developed for detecting staphylococcal enterotoxin A in food products. Sample preparation can be completed in less than 15 min. Assay sensitivity ranges from 0.4 ng (20-h test time) to 3.2 ng (1- to 3-h test time) of toxin per ml of prepared sample. Separation and detection of enterotoxin from spiked food products ranged between 72 and 98% of the amount added.
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Selected References
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- CASMAN E. P., BENNETT R. W. DETECTION OF STAPHYLOCOCCAL ENTEROTOXIN IN FOOD. Appl Microbiol. 1965 Mar;13:181–189. doi: 10.1128/am.13.2.181-189.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Collins W. S., 2nd, Metzger J. F., Johnson A. D. A rapid solid phase radioimmunoassay for staphylococcal B enterotoxin. J Immunol. 1972 Mar;108(3):852–856. [PubMed] [Google Scholar]
- Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
- Genigeorgis C., Kuo J. K. Recovery of staphylococcal enterotoxin from foods by affinity chromatography. Appl Environ Microbiol. 1976 Feb;31(2):274–279. doi: 10.1128/aem.31.2.274-279.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Johnson H. M., Bukovic J. A., Kauffmann P. E. Staphylococcal enterotoxins A and B: solid-phase radioimmunoassay in food. Appl Microbiol. 1973 Sep;26(3):309–313. doi: 10.1128/am.26.3.309-313.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nakane P. K., Kawaoi A. Peroxidase-labeled antibody. A new method of conjugation. J Histochem Cytochem. 1974 Dec;22(12):1084–1091. doi: 10.1177/22.12.1084. [DOI] [PubMed] [Google Scholar]
- Saunders G. C., Clinard E. H. Rapid micromethod of screening for antibodies to disease agents using the indirect enzyme-labeled antibody test. J Clin Microbiol. 1976 Jun;3(6):604–608. doi: 10.1128/jcm.3.6.604-608.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Saunders G. C. Development and evaluation of an enzyme-labeled antibody test for the rapid detection of hog cholera antibodies. Am J Vet Res. 1977 Jan;38(1):21–25. [PubMed] [Google Scholar]
- Silverman S. J., Knott A. R., Howard M. Rapid, sensitive assay for staphylococcal enterotoxin and a comparison of serological methods. Appl Microbiol. 1968 Jul;16(7):1019–1023. doi: 10.21236/ad0838753. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Van Weemen B. K., Schuurs A. H.W.M. Immunoassay using antigen-enzyme conjugates. FEBS Lett. 1971 Jun 24;15(3):232–236. doi: 10.1016/0014-5793(71)80319-8. [DOI] [PubMed] [Google Scholar]
- Voller A., Bidwell D. E., Bartlett A. Enzyme immunoassays in diagnostic medicine. Theory and practice. Bull World Health Organ. 1976;53(1):55–65. [PMC free article] [PubMed] [Google Scholar]
- Wolters G., Kuijpers L., Kacaki J., Schuurs A. Solid-phase enzyme-immunoassay for detection of hepatitis B surface antigen. J Clin Pathol. 1976 Oct;29(10):873–879. doi: 10.1136/jcp.29.10.873. [DOI] [PMC free article] [PubMed] [Google Scholar]