Abstract
A strain of Spirillum lipoferum with intense red pigmentation was isolated from the roots of Cynodon dactylon "Coastal." This isolate vigorously reduced acetylene when grown in N-free, Na-malate, semisolid agar, and it was identical to S. lipoferum strain SP7 by standard taxonomic tests. Both S. lipoferum SP7 and the C. dactylon root isolate displayed the unique features of being denitrifiers as well as N2 fixers. The N2-dependent growth curve was biphasic: cells in younger cultures showed the characteristic spiral shape and motility, but those in older cultures developed larger, nonmotile, cystlike forms. Nitrogenase activity seemed associated only with younger spiral forms. The red pigment may be a b- or c-type cytochrome. The strong red color, which this strain develops, could be used as a marker in evaluating soil inoculation experiments.
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- Barber L. E., Evans H. J. Characterization of a nitrogen-fixing bacterial strain from the roots of Digitaria sanguinalis. Can J Microbiol. 1976 Feb;22(2):254–260. doi: 10.1139/m76-034. [DOI] [PubMed] [Google Scholar]
- MECHSNER K., WUHRMANN K. BEITRAG ZUR KENNTNIS DER MIKROBIELLEN DENITRIFIKATION. Pathol Microbiol (Basel) 1963;26:579–591. [PubMed] [Google Scholar]
- Neyra C. A., Döbereiner J. Denitrification by N2-fixing Sprillum lipoferum. Can J Microbiol. 1977 Mar;23(3):300–305. doi: 10.1139/m77-044. [DOI] [PubMed] [Google Scholar]
- Okon Y., Albrecht S. L., Burris R. H. Factors affecting growth and nitrogen fixation of Spirillum lipoferum. J Bacteriol. 1976 Sep;127(3):1248–1254. doi: 10.1128/jb.127.3.1248-1254.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Payne W. J. Reduction of nitrogenous oxides by microorganisms. Bacteriol Rev. 1973 Dec;37(4):409–452. doi: 10.1128/br.37.4.409-452.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith R. L., Bouton J. H., Schank S. C., Quesenberry K. H., Tyler M. E., Milam J. R., Gaskins M. H., Littell R. C. Nitrogen Fixation in Grasses Inoculated with Spirillum lipoferum. Science. 1976 Sep 10;193(4257):1003–1005. doi: 10.1126/science.193.4257.1003. [DOI] [PubMed] [Google Scholar]
- Von Bülow J. F., Döbereiner J. Potential for nitrogen fixation in maize genotypes in Brazil. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2389–2393. doi: 10.1073/pnas.72.6.2389. [DOI] [PMC free article] [PubMed] [Google Scholar]