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. 1977 Nov;34(5):591–594. doi: 10.1128/aem.34.5.591-594.1977

Electron microscopy of Mycoplasma pneumoniae microcolonies grown on solid surfaces.

C K Kim, R M Pfister, N L Somerson
PMCID: PMC242706  PMID: 931378

Abstract

Mycoplasma pneumoniae sprain CL-8 was studied by using various surfaces for adherence and growth. Cells grown on Epon 812, Formvar, carbon, and glass were of similar morphology. Thin Epon pieces were good material for culturing the organisms and examining thin-sectioned microcolonies by transmission electron microscopy.

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Selected References

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  1. BRADLEY D. E., WILLIAMS D. J. An electron microscope study of the spores of some species of the genus Bacillus using carbon replicas. J Gen Microbiol. 1957 Aug;17(1):75–79. doi: 10.1099/00221287-17-1-75. [DOI] [PubMed] [Google Scholar]
  2. Biberfeld G., Biberfeld P. Ultrastructural features of Mycoplasma pneumoniae. J Bacteriol. 1970 Jun;102(3):855–861. doi: 10.1128/jb.102.3.855-861.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boatman E. S., Kenny G. E. Morphology and ultrastructure of Mycoplasma pneumoniae spherules. J Bacteriol. 1971 Jun;106(3):1005–1015. doi: 10.1128/jb.106.3.1005-1015.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Boatman E. S. Morphologic heterogeneity of the Mycoplasmatales. J Infect Dis. 1973 Mar;127(Suppl):S12–S14. doi: 10.1093/infdis/127.supplement_1.s12. [DOI] [PubMed] [Google Scholar]
  5. Bruch J., Sethi K. K. Electron microscopy of ultrathin sections of Mycoplasma gallisepticum (strain TT) organisms grown on glass surface. Pathol Microbiol (Basel) 1973;39(5):373–382. doi: 10.1159/000162676. [DOI] [PubMed] [Google Scholar]
  6. Luft J. H. Ruthenium red and violet. II. Fine structural localization in animal tissues. Anat Rec. 1971 Nov;171(3):369–415. doi: 10.1002/ar.1091710303. [DOI] [PubMed] [Google Scholar]
  7. Powell D. A., Hu P. C., Wilson M., Collier A. M., Baseman J. B. Attachment of Mycoplasma pneumoniae to respiratory epithelium. Infect Immun. 1976 Mar;13(3):959–966. doi: 10.1128/iai.13.3.959-966.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Purcell R. H., Valdesuso J. R., Cline W. L., James W. D., Chanock R. M. Cultivation of Mycoplasmas on glass. Appl Microbiol. 1971 Feb;21(2):288–294. doi: 10.1128/am.21.2.288-294.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Wilson M. H., Collier A. M. Ultrastructural study of Mycoplasma pneumoniae in organ culture. J Bacteriol. 1976 Jan;125(1):332–339. doi: 10.1128/jb.125.1.332-339.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

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