Skip to main content
Applied and Environmental Microbiology logoLink to Applied and Environmental Microbiology
. 1977 Dec;34(6):626–629. doi: 10.1128/aem.34.6.626-629.1977

Effect of growth substrate on thermal death of thermophilic bacteria.

G J Merkel, J J Perry
PMCID: PMC242721  PMID: 596868

Abstract

The heat sensitivity of gram-negative, hydrocarbon-utilizing thermophilic bacteria was altered by a change in growth substrate. Thermophilic strains CC-6, BI-1, and LEH-1, grown with acetate or n-heptadecane as the carbon source, had a higher survival rate when incubated 5 degrees C above their maximum growth temperature than cells of the same organism after growth on glucose or glycerol. There was a correlation between the growth substrated, heat resistance, and the ratios of cellular n-hexadecanoic acid/branched hexadecanoic acid and n-heptadecanoic acid/branched heptadecanoic acid. The bacterial cells that were more heat resistant had ratios of straight-chain/branched-chain fatty acids above 1.0, whereas the heat-sensitive cells had ratios below 0.6.

Full text

PDF
626

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bauman A. J., Simmonds P. G. Fatty acids and polar lipids of extremely thermophilic filamentous bacterial masses from two Yellowstone hot springs. J Bacteriol. 1969 May;98(2):528–531. doi: 10.1128/jb.98.2.528-531.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brock T. D. Life at high temperatures. Evolutionary, ecological, and biochemical significance of organisms living in hot springs is discussed. Science. 1967 Nov;158(3804):1012–1019. doi: 10.1126/science.158.3804.1012. [DOI] [PubMed] [Google Scholar]
  3. Campbell L. L., Pace B. Physiology of growth at high temperatures. J Appl Bacteriol. 1968 Mar;31(1):24–35. doi: 10.1111/j.1365-2672.1968.tb00338.x. [DOI] [PubMed] [Google Scholar]
  4. Cerniglia C. E., Perry J. J. Effect of substrate on the fatty acid composition of hydrocarbon-utilizing filamentous fungi. J Bacteriol. 1974 Jun;118(3):844–847. doi: 10.1128/jb.118.3.844-847.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cronan J. E., Jr, Gelmann E. P. Physical properties of membrane lipids: biological relevance and regulation. Bacteriol Rev. 1975 Sep;39(3):232–256. doi: 10.1128/br.39.3.232-256.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DAVIS J. B. MICROBIAL INCORPORATION OF FATTY ACIDS DERIVED FROM N-ALKANES INTO GLYCERIDES AND WAXES. Appl Microbiol. 1964 May;12:210–214. doi: 10.1128/am.12.3.210-214.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dunlap K. R., Perry J. J. Effect of Substrate on the Fatty Acid Composition of Hydrocarbon- and Ketone-utilizing Microorganisms. J Bacteriol. 1968 Aug;96(2):318–321. doi: 10.1128/jb.96.2.318-321.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dunlap K. R., Perry J. J. Effect of substrate on the fatty acid composition of hydrocabon-utilizing microorganisms. J Bacteriol. 1967 Dec;94(6):1919–1923. doi: 10.1128/jb.94.6.1919-1923.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Farrell J., Rose A. Temperature effects on microorganisms. Annu Rev Microbiol. 1967;21:101–120. doi: 10.1146/annurev.mi.21.100167.000533. [DOI] [PubMed] [Google Scholar]
  10. Gill C. O. Effect of growth temperature on the lipids of Pseudomonas fluorescens. J Gen Microbiol. 1975 Aug;89(2):293–298. doi: 10.1099/00221287-89-2-293. [DOI] [PubMed] [Google Scholar]
  11. Heinen W. Growth conditions and temperature-dependent substrate specificity of two extremely thermophilic bacteria. Arch Mikrobiol. 1971;76(1):2–17. doi: 10.1007/BF00409310. [DOI] [PubMed] [Google Scholar]
  12. Heinen W., Klein H. P., Volkmann C. M. Fatty acid composition of Thermus aquaticus at different growth temperatures. Arch Mikrobiol. 1970;72(2):199–202. doi: 10.1007/BF00409525. [DOI] [PubMed] [Google Scholar]
  13. Kaneda T. Positional preference of fatty acids in phospholipids of Bacillus cereus and its relation to growth temperature. Biochim Biophys Acta. 1972 Oct 5;280(2):297–305. doi: 10.1016/0005-2760(72)90097-5. [DOI] [PubMed] [Google Scholar]
  14. King D. H., Perry J. J. The origin of fatty acids in the hydrocarbon-utilizing microorganism Mycobacterium vaccae. Can J Microbiol. 1975 Jan;21(1):85–89. doi: 10.1139/m75-012. [DOI] [PubMed] [Google Scholar]
  15. LEADBETTER E. R., FOSTER J. W. Studies on some methane-utilizing bacteria. Arch Mikrobiol. 1958;30(1):91–118. doi: 10.1007/BF00509229. [DOI] [PubMed] [Google Scholar]
  16. Makula R., Finnerty W. R. Microbial assimilation of hydrocarbons. I. Fatty acids derived from normal alkanes. J Bacteriol. 1968 Jun;95(6):2102–2107. doi: 10.1128/jb.95.6.2102-2107.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mosley G. A., Card G. L., Koostra W. L. Effect of calcium and anaerobiosis on the thermostability of Bacillus stearothermophilus. Can J Microbiol. 1976 Apr;22(4):468–474. doi: 10.1139/m76-073. [DOI] [PubMed] [Google Scholar]
  18. Nicolaides N., Fu H. C. A rapid micro technique for differentiating between iso, anteiso and other mono methyl branched fatty chains. Lipids. 1969 Jan;4(1):83–86. doi: 10.1007/BF02531802. [DOI] [PubMed] [Google Scholar]
  19. Oshima M., Miyagawa A. Comparative studies on the fatty acid composition of moderately and extremely thermophilic bacteria. Lipids. 1974 Jul;9(7):476–480. doi: 10.1007/BF02534274. [DOI] [PubMed] [Google Scholar]
  20. Ray P. H., White D. C., Brock T. D. Effect of temperature on the fatty acid composition of Thermus aquaticus. J Bacteriol. 1971 Apr;106(1):25–30. doi: 10.1128/jb.106.1.25-30.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Singleton R., Jr, Amelunxen R. E. Proteins from thermophilic microorganisms. Bacteriol Rev. 1973 Sep;37(3):320–342. doi: 10.1128/br.37.3.320-342.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Souza K. A., Kostiw L. L., Tyson B. J. Alterations in normal fatty acid composition in a temperature-sensitive mutant of a thermophilic bacillus. Arch Microbiol. 1974 Apr 19;97(2):89–102. doi: 10.1007/BF00403049. [DOI] [PubMed] [Google Scholar]
  23. Weerkamp A., Heinen W. Effect of temperature on the fatty acid composition of the extreme thermophiles, Bacillus caldolyticus and Bacillus caldotenax. J Bacteriol. 1972 Jan;109(1):443–446. doi: 10.1128/jb.109.1.443-446.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Yao M., Walker H. W., Lillard D. A. Fatty acids from vegetative cells and spores of Bacillus stearothermophilus. J Bacteriol. 1970 Jun;102(3):877–878. doi: 10.1128/jb.102.3.877-878.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES