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. 1969;41(2):269–273.

Enzymes of Entamoeba histolytica

Rawewan Jarumilinta, B G Maegraith
PMCID: PMC2427427  PMID: 4312971

Abstract

In spite of extensive studies of the pathogenic activities of Entamoeba histolytica upon animal hosts by many investigators, the factors that govern the pathogenesis of the parasite are still largely unknown. Attempts have been made to determine the factors concerned in the pathogenicity of certain strains of E. histolytica from the aspect of parasite itself and from the point of view of its interaction with the host. The enzyme content of the trophozoites of the amoebae examined has been studied in detail, both in the living organism and in its extracts. The total patterns of proteolytic enzymes and of certain other enzymes were determined to establish whether it was possible to define the invasiveness of the various strains of E. histolytica already established as pathogenic by other workers or in our laboratories. It was found impossible to distinguish clearly between the so-called ”pathogenic” and ”non-pathogenic” strains of E. histolytica by such means.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAERNSTEIN H. D., REES C. W., REARDON L. V. Symbiosis in cultures of Endamoeba histolytica and single species of bacteria. Am J Trop Med Hyg. 1954 Sep;3(5):839–848. doi: 10.4269/ajtmh.1954.3.839. [DOI] [PubMed] [Google Scholar]
  2. BLUMENTHAL H., MICHAELSON J. B., DELAMATER J. N. Some aspects of the phosphomonoesterase activity of Endamoeba histolytica. Exp Parasitol. 1955 May;4(3):201–207. doi: 10.1016/0014-4894(55)90025-4. [DOI] [PubMed] [Google Scholar]
  3. DELAMATER J. N., MICHAELSON J. B., HALLMAN F. A., BLUMENTHAL H. An investigation into hyaluronidase as a factor in the mechanism of tissue invasion by Endamoeba histolytica. Am J Trop Med Hyg. 1954 Jan;3(1):1–8. doi: 10.4269/ajtmh.1954.3.1. [DOI] [PubMed] [Google Scholar]
  4. HALLMAN F. A., MICHAELSON J. B., DeLAMATER J. N. The cultivation of Endamoeba histolytica in a defined medium. Am J Trop Med Hyg. 1950 May;30(3):363–369. doi: 10.4269/ajtmh.1950.s1-30.363. [DOI] [PubMed] [Google Scholar]
  5. HARINASUTA C., MAEGRAITH B. G. The demonstration of proteolytic enzyme activity of Entamoeba histolytica by the use of photographic gelatin film. Ann Trop Med Parasitol. 1958 Dec;52(4):508–515. doi: 10.1080/00034983.1958.11685888. [DOI] [PubMed] [Google Scholar]
  6. HILKER D. M., SHERMAN H. J., WHITE A. G. Starch hydrolysis by Entamoeba histolytica. Exp Parasitol. 1957 Sep;6(5):459–464. doi: 10.1016/0014-4894(57)90034-6. [DOI] [PubMed] [Google Scholar]
  7. JARUMILINTA R., KRADOLFER F. THE TOXIC EFFECT OF ENTAMOEBA HISTOLYTICA ON LEUCOCYTES. Ann Trop Med Parasitol. 1964 Sep;58:375–381. doi: 10.1080/00034983.1964.11686259. [DOI] [PubMed] [Google Scholar]
  8. JARUMILINTA R., MAEGRAITH B. G. Hyaluronidase activity in stock cultures of Entamoeba histolytica. Ann Trop Med Parasitol. 1960 Apr;54:118–128. doi: 10.1080/00034983.1960.11685965. [DOI] [PubMed] [Google Scholar]
  9. JARUMILINTA R., MAEGRAITH B. G. The patterns of some proteolytic enzymes of Entamoeba histolytica and Acanthamoeba sp. I. The action of E. histolytica and Acanthamoeba sp. on protein substrates. Ann Trop Med Parasitol. 1961 Dec;55:505–517. doi: 10.1080/00034983.1961.11686080. [DOI] [PubMed] [Google Scholar]
  10. JARUMILINTA R., MAEGRAITH B. G. The patterns of some proteolytic enzymes of Entamoeba histolytica and Acanthamoeba sp. II. The action E. histolytica and acanthamoeba sp. on various synthetic substrates. Ann Trop Med Parasitol. 1961 Dec;55:518–528. [PubMed] [Google Scholar]
  11. Meyer K., Chaffee E., Hobby G. L., Dawson M. H. HYALURONIDASES OF BACTERIAL AND ANIMAL ORIGIN. J Exp Med. 1941 Feb 28;73(3):309–326. doi: 10.1084/jem.73.3.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. NAKAMURA M., EDWARDS P. R., Jr Casease in Entamoeba histolytica. Nature. 1959 Feb 7;183(4658):397–397. doi: 10.1038/183397b0. [DOI] [PubMed] [Google Scholar]
  13. NAKAMURA M., GOLDSTEIN L. Occurrence of glutaminase in Endamoeba histolytica. Nature. 1957 Jun 1;179(4570):1134–1134. doi: 10.1038/1791134a0. [DOI] [PubMed] [Google Scholar]
  14. NEAL R. A. Enzymic proteolysis by Entamoeba histolytica; biochemical characteristics and relationship with invasiveness. Parasitology. 1960 Nov;50:531–550. doi: 10.1017/s0031182000025610. [DOI] [PubMed] [Google Scholar]
  15. NEAL R. A. Proteolytic enzymes in Entamoeba histolytica. Nature. 1956 Sep 15;178(4533):599–599. doi: 10.1038/178599a0. [DOI] [PubMed] [Google Scholar]
  16. PERMIN P. M. Two simple methods of determining fibrinolytic enzymes. Acta Physiol Scand. 1950 May 30;20(4):388–393. doi: 10.1111/j.1748-1716.1950.tb00717.x. [DOI] [PubMed] [Google Scholar]
  17. REES C. W., BAERNSTEIN H. D., REARDON L. V., PHILLIPS L. Some interactions in vitro of Endamoeba histolytica and single species of microbial symbionts. Am J Trop Med Hyg. 1953 Nov;2(6):1002–1014. doi: 10.4269/ajtmh.1953.2.1002. [DOI] [PubMed] [Google Scholar]
  18. SEAMAN G. R. Inhibition of the succinic dehydrogenase of parasitic protozoans by an arsono and a phosphono analog of succinic acid. Exp Parasitol. 1953 Oct;2(4):366–373. doi: 10.1016/0014-4894(53)90022-8. [DOI] [PubMed] [Google Scholar]
  19. SMITH I. Colour reactions on paper chromatograms by a dipping technique. Nature. 1953 Jan 3;171(4340):43–44. doi: 10.1038/171043a0. [DOI] [PubMed] [Google Scholar]
  20. Swyer G. I., Emmens C. W. A modified method for the viscosimetric assay of hyaluronidase. Biochem J. 1947;41(1):29–34. doi: 10.1042/bj0410029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. THIRST M. L. Gelatin liquefaction: a microtest. J Gen Microbiol. 1957 Oct;17(2):396–400. doi: 10.1099/00221287-17-2-396. [DOI] [PubMed] [Google Scholar]

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