Abstract
Cultivation of Mycobacterium lepraemurium in cell-free media has not yet been successful, perhaps because of deterioration of the nutritional quality of the medium with prolonged incubation at 37°C. Trials with a continuous culture technique, involving regular change of a simple liquid medium prepared from human placenta, demonstrated active fission of the Myco. lepraemurium cells. Abundant growth in the form by macroscopically visible colonies has not been achieved even after prolonged incubation up to 8 months.
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- HANKS J. H., GRAY C. T. The application of metabolic studies to leprosy research. Int J Lepr. 1954 Apr-Jun;22(2):147–161. [PubMed] [Google Scholar]
- HART P. A., VALENTINE R. C. Elongation of a leprosy bacillus (Mycobacterium lepraemurium) in a cell-free medium. Nature. 1960 Jan 2;185:58–60. doi: 10.1038/185058a0. [DOI] [PubMed] [Google Scholar]
- REES R. J., WONG P. C. Limited multiplication of M. lepraemurium in tissue culture. Nature. 1958 Feb 1;181(4605):359–360. doi: 10.1038/181359b0. [DOI] [PubMed] [Google Scholar]
- SULA L. WHO CO-OPERATIVE STUDIES ON A SIMPLE CULTURE TECHNIQUE FOR THE ISOLATION OF MYCOBACTERIA. 1. PREPARATION, LYOPHILIZATION AND RECONSTITUTION OF A SIMPLE SEMI-SYNTHETIC CONCENTRATED LIQUID MEDIUM; CULTURE TECHNIQUE; GROWTH PATTERN OF DIFFERENT MYCOBACTERIA. Bull World Health Organ. 1963;29:589–606. [PMC free article] [PubMed] [Google Scholar]