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. 1978 Feb;35(2):228–230. doi: 10.1128/aem.35.2.228-230.1978

Selective enrichment of aromatic amino acid auxotrophs in Hansenula polymorpha.

S Sanchez, A Cea, M E Flores
PMCID: PMC242816  PMID: 345964

Abstract

Aromatic amino acid auxotrophs of the methanol-utilizing yeast Hansenula polymorpha were effectively selected by the use of nystatin and a medium that inhibits the growth of tyrosine auxotrophs. The procedure resulted in a frequency of aromatic auxotrophs of 2% of survivors and an enrichment of 20-fold. The new procedure also takes less time than traditional procedures. Of the auxotrophic mutants isolated, two-thirds required tyrosine and the remainder were tyrosine-phenylalanine double auxotrophs.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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