Abstract
A simple test for the serological separation of staphylococci from micrococci is described, which is based on the quite different cell wall peptidoglycan structures of these two genera. Antisera to (pentaglycyl-epsilon-amino-n-hexanoic acid)20-albumin agglutinated without exception all staphylococci and gave no positive reaction with micrococci or other bacterial cells. To obtain a good reaction, it was necessary to extract the cells with hot trichloroacetic acid for 30 min. Antisera to (tri-L-alanyl-epsilon-amino-n-hexanoic acid)22-albumin reacted strongly with micrococci containing oligo-L-alanine bridges in their peptidoglycan, but did not agglutinate staphylococci or other bacteria lacking alanine interpeptide bridges.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BAIRD-PARKER A. C. A classification of micrococci and staphylococci based on physiological and biochemical tests. J Gen Microbiol. 1963 Mar;30:409–427. doi: 10.1099/00221287-30-3-409. [DOI] [PubMed] [Google Scholar]
- Evans J. B., Kloos W. E. Use of shake cultures in a semisolid thioglycolate medium for differentiating staphylococci from micrococci. Appl Microbiol. 1972 Feb;23(2):326–331. doi: 10.1128/am.23.2.326-331.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schechter B., Schechter I., Sela M. Antibody combining sites to a series of peptide determinants of increasing size and defined structure. J Biol Chem. 1970 Mar 25;245(6):1438–1447. [PubMed] [Google Scholar]
- Schleifer K. H., Kandler O. Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev. 1972 Dec;36(4):407–477. doi: 10.1128/br.36.4.407-477.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schleifer K. H., Seidl P. H. The immunochemistry of peptidoglycan. Antibodies against a synthetic immunogen cross-reacting with peptidoglycan. Eur J Biochem. 1974 Apr 16;43(3):509–519. doi: 10.1111/j.1432-1033.1974.tb03438.x. [DOI] [PubMed] [Google Scholar]
- Seidl P. H., Schleifer K. H. The immunochemistry of peptidoglycan. Antibodies against a synthetic immunogen cross-reacting with an interpeptide bridge of peptidoglycan. Eur J Biochem. 1977 Apr 1;74(2):353–363. doi: 10.1111/j.1432-1033.1977.tb11400.x. [DOI] [PubMed] [Google Scholar]