Abstract
The NaCl tolerance of different strains of Clostridium botulinum varies over a wide range, and the patterns of NaCl inhibition differ distinctly and characteristically from strain to strain. The more radiation-resistant strains, such as 33A, 62A, and 7272A, are more resistant to NaCl, whereas the more radiation-sensitive strains, such as 51B and 1304E, are more sensitive to NaCl. This rule appears to hold irrespective of whether the spores were unirradiated controls or whether they were radiation damaged prior to exposure to NaCl in the recovery media. The data seem to indicate that radiation doses in the shoulder portion of the radiation survival curves did not noticeably sensitive the spores to NaCl, whereas radiation doses in the exponential-decline portion of the survival curve invariably produced a distinct sensitization. Thus, strains 33A and 62A were not sensitized to NaCl by 0.3 to 0.4 Mrad, i.e., in the shoulder portion of the survival curve. Radiation-sensitive strain 51B, which shows no distinct shoulder in its survival curve, was sensitized to NaCl by 0.1 Mrad, the lowest radiation dose employed in this study. These observations seem to suggest a possible relationship between deoxyribonucleic acid repair capacity and salt tolerance.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ANELLIS A., KOCH R. B. Comparative resistance of strains of Clostridium botulinum to gamma rays. Appl Microbiol. 1962 Jul;10:326–330. doi: 10.1128/am.10.4.326-330.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Anellis A., Shattuck E., Rowley D. B., Ross E. W., Jr, Whaley D. N., Dowell V. R., Jr Low-temperature irradiation of beef and methods of evaluation of radappertization process. Appl Microbiol. 1975 Nov;30(5):811–820. doi: 10.1128/am.30.5.811-820.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Driedger A. A., Grayston M. J. Demonstration of two types of DNA repair in X-irradiated Micrococcus radiodurans. Can J Microbiol. 1971 Apr;17(4):495–499. doi: 10.1139/m71-082. [DOI] [PubMed] [Google Scholar]
- Durban E., Durban E. M., Grecz N. Production of spore spheroplasts of Clostridium botulinum and DNA extraction for density gradient centrifugation. Can J Microbiol. 1974 Mar;20(3):353–358. doi: 10.1139/m74-054. [DOI] [PubMed] [Google Scholar]
- Durban E., Grecz N., Farkas J. Direct enzymatic repair of deoxyribonucleic acid single-strand breaks in dormant spores. J Bacteriol. 1974 Apr;118(1):129–138. doi: 10.1128/jb.118.1.129-138.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Durban E., Grecz N. Resistance of spores of Clostridium botulinum 33A to combinations of ultraviolet and gamma rays. Appl Microbiol. 1969 Jul;18(1):44–50. doi: 10.1128/am.18.1.44-50.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GRECZ N., ANELLIS A., SCHNEIDER M. D. Procedure for cleaning of Clostridium botulinum spores. J Bacteriol. 1962 Sep;84:552–558. doi: 10.1128/jb.84.3.552-558.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hansen J. N., Spiegelman G., Halvorson H. O. Bacterial spore outgrowth: its regulation. Science. 1970 Jun 12;168(3937):1291–1298. doi: 10.1126/science.168.3937.1291. [DOI] [PubMed] [Google Scholar]
- Lehman I. R. DNA ligase: structure, mechanism, and function. Science. 1974 Nov 29;186(4166):790–797. doi: 10.1126/science.186.4166.790. [DOI] [PubMed] [Google Scholar]
- Roberts T. A., Ditchett P. J., Ingram M. The effect of sodium chloride on radiation resistance and recovery of irradiated anaerobic spores. J Appl Bacteriol. 1965 Aug;28(2):336–348. doi: 10.1111/j.1365-2672.1965.tb02162.x. [DOI] [PubMed] [Google Scholar]