Abstract
Continuous culture of Bacillus popilliae was achieved for the first time in a small chemostat. Initially, variable cell yields during steady-state chemostat growth led to a re-examination of growth rates in batch cultures. B. popilliae NRRL B-2309 and a wild strain were both found to be natural mixtures of three substrains characterized by different growth rates and colony morphologies and varying stability. Selected subcultures grown continuously provided data for three different cell production curves. Cell yields were two to three times greater per unit of medium in continuous than in batch culture, and about 1% of slow-growing chemostat cells formed typical spores.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Haynes W. C., Rhodes L. J. Spore formation by Bacillus popilliae in liquid medium containing activated carbon. J Bacteriol. 1966 Jun;91(6):2270–2274. doi: 10.1128/jb.91.6.2270-2274.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rhodes R. A., Roth M. S., Hrubant G. R. Sporulation of bacillus popilliae on solid media. Can J Microbiol. 1965 Oct;11(5):779–783. doi: 10.1139/m65-105. [DOI] [PubMed] [Google Scholar]
- STEINKRAUS K. H. Studies on the milky disease organisms. I. Parasitic growth and sporulation of Bacillus popilliae. J Bacteriol. 1957 Nov;74(5):621–624. doi: 10.1128/jb.74.5.621-624.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SYLVESTER C. J., COSTILOW R. N. NUTRITIONAL REQUIREMENTS OF BACILLUS POPILLIAE. J Bacteriol. 1964 Jan;87:114–119. doi: 10.1128/jb.87.1.114-119.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sharpe E. S., St Julian G., Crowell C. Characteristics of a new strain of Bacillus popilliae sporogenic in vitro. Appl Microbiol. 1970 Apr;19(4):681–688. doi: 10.1128/am.19.4.681-688.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- St Julian G., Hall H. H. Infection of Popillia japonica larvae with heat-activated spores of Bacillus popilliae. J Invertebr Pathol. 1968 Feb;10(1):48–53. doi: 10.1016/0022-2011(68)90262-0. [DOI] [PubMed] [Google Scholar]
- Wyss C. Sporulationsversuche mit drei Varietäten von Bacillus popilliae Dutky. Zentralbl Bakteriol Parasitenkd Infektionskr Hyg. 1971;126(5):461–492. [PubMed] [Google Scholar]