Abstract
The survival, recovery, and identification of Streptococcus isolates from soil was investigated by (i) examination of survival in soil under different moisture and temperature conditions, (ii) evaluation of media combinations for recovering fecal streptococci from soil, and (iii) partial identification of isolates from diverse habitats. Cool, moist conditions prolonged the survival of Streptococcus faecalis in soil for at least 12 weeks, whereas freezing was lethal, with the populations being reduced up to 95% when several freeze-thaw treatments occurred. Media evaluations indicated that both the efficiency of recovery and enumeration of the fecal streptococci from soil can be influenced by the combination of media used. Taxonomic data revealed a need to develop procedures to differentiate between isolates of fecal origin and plant-derived streptococci that possess many of the cultural reactions of S. faecalis. It was found that recent fecal isolates exhibited a much greater incidence of multiple antibiotic resistance than soil or vegetation isolates, and this characteristic, coupled with the use of enterococci as indicators of fecal contamination in soil systems, is discussed.
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Selected References
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- Facklam R. R. Recognition of group D streptococcal species of human origin by biochemical and physiological tests. Appl Microbiol. 1972 Jun;23(6):1131–1139. doi: 10.1128/am.23.6.1131-1139.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Geldreich E. E., Kenner B. A. Concepts of fecal streptococci in stream pollution. J Water Pollut Control Fed. 1969 Aug;41(8 Suppl):R336+–R336+. [PubMed] [Google Scholar]
- Hagedorn C. Influences of soil acidity on Streptomyces populations inhabiting forest soils. Appl Environ Microbiol. 1976 Sep;32(3):368–375. doi: 10.1128/aem.32.3.368-375.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hartman P. A., Reinbold G. W., Saraswat D. S. Media and methods for isolation and enumeration of the enterococci. Adv Appl Microbiol. 1966;8:253–289. [PubMed] [Google Scholar]
- MUNDT J. O. Occurrence of enterococci on plants in a wild environment. Appl Microbiol. 1963 Mar;11:141–144. doi: 10.1128/am.11.2.141-144.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mundt J. O., Graham W. F., McCarty I. E. Spherical Lactic Acid-producing Bacteria of Southern-grown Raw and Processed Vegetables. Appl Microbiol. 1967 Nov;15(6):1303–1308. doi: 10.1128/am.15.6.1303-1308.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sherman J. M. THE STREPTOCOCCI. Bacteriol Rev. 1937 Dec;1(1):3–97. doi: 10.1128/br.1.1.3-97.1937. [DOI] [PMC free article] [PubMed] [Google Scholar]