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. 1978 May;35(5):878–880. doi: 10.1128/aem.35.5.878-880.1978

Affinity chromatography purification of type A botulinum neurotoxin from crystalline toxic complex.

L J Moberg, H Sugiyama
PMCID: PMC242946  PMID: 655704

Abstract

Type A botulinum neurotoxin was purified from toxic crystals by adsorption to p-aminophenyl-beta-D-thiogalactopyranoside coupled to CH-Sepharose 4B. At pH 6.3, the toxic complex was held by the binding between the ligand and the hemagglutinin of the complex; the toxin is eluted selectively by dissociating the complex with buffer-saline of pH 7.9. The single-step affinity chromatography recovered 50 to 60% of applied toxicity as preparations of greater than 99% purity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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