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. 1978 May;35(5):890–896. doi: 10.1128/aem.35.5.890-896.1978

Kinetics of biodegradation of p-nitrobenzoate and inhibition by benzoate in a pseudomonad.

H D Haller, R K Finn
PMCID: PMC242949  PMID: 655705

Abstract

The degradation of p-nitrobenzoate (p-NBA) by domestic sewage was inhibited by benzoate, and a model for this behavior was found in a soil isolate. The isolate, a pseudomonad, utilized p-NBA and benzoate by separate adaptive enzyme pathways. In oxygen uptake experiments, the degradation of p-NBA was competitively inhibited by benzoate, but the degradation of benzoate was not affected by the presence of p-NBA. 4-Nitrocatechol was not implicated in the inhibition. p-Hydroxybenzoate, which is the p-NBA degradation pathway, also had a decreased rate od degradation when benzoate was present. The growth rate of the isolate on the aromatic substrates and on glucose autoclaved in the medium was 0.3 h-1. When glucose was autoclaved separately, the growth rate was less, about 0.2 h-1. The apparent Km in oxygen uptake experiments was 25 micrometer for p-NBA and benzoate and 5 micrometer for p-hydroxybenzoate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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