Abstract
The influence of the inclusion of xylan in a medium for enumeration of total culturable rumen bacteria was investigated. Maximum colony numbers were obtained on a medium, GCSX-2, which contained 0.033% each glucose and cellobiose and 0.067% each soluble starch and xylan. This medium gave higher colony counts than either medium 98-5 of Bryant and Robinson (J. Dairy Sci. 44:1446-1456, 1961), medium 98-5 of Chung and Hungate (Appl. Environ. Microbiol. 32:649-652, 1976), containing an added lucerne (hemicellulose + cellulose) fiber substrate, or medium GCSX-2 with the added lucerne (hemicellulose + cellulose) fraction. The time of collection of rumen fluid influenced the colony counts on the media containing the lucerne fiber substrate but was without effect on medium GCSX-2.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Chung K. T., Hungate R. E. Effect of alfalfa fiber substrate on culture counts of rumen bacteria. Appl Environ Microbiol. 1976 Oct;32(4):649–652. doi: 10.1128/aem.32.4.649-652.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dehority B. A., Grubb J. A. Basal medium for the selective enumeration of rumen bacteria utilizing specific energy sources. Appl Environ Microbiol. 1976 Nov;32(5):703–710. doi: 10.1128/aem.32.5.703-710.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dehority B. A. Hemicellulose degradation by rumen bacteria. Fed Proc. 1973 Jul;32(7):1819–1825. [PubMed] [Google Scholar]
- Grubb J. A., Dehority B. A. Variation in colony counts of total viable anaerobic rumen bacteria as influenced by media and cultural methods. Appl Environ Microbiol. 1976 Feb;31(2):262–267. doi: 10.1128/aem.31.2.262-267.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van Gylswyk N. O. A comparison of two techniques for counting cellulolytic rumen bacteria. J Gen Microbiol. 1970 Feb;60(2):191–197. doi: 10.1099/00221287-60-2-191. [DOI] [PubMed] [Google Scholar]
- van Gylswyk N. O., Hoffman J. P. Characteristics of cellulolytic cillobacteria from the rumens of sheep fed teff (Eragrostis tef) hay diets. J Gen Microbiol. 1970 Mar;60(3):381–386. doi: 10.1099/00221287-60-3-381. [DOI] [PubMed] [Google Scholar]