Figure 2.—
InR/TOR signaling controls the differentiation of specific cell types in the developing eye. (A and A′) Cells mutant for tsc1 (tsc1Q87X) show precocious differentiation of PRs 1 and 6 (stained for Bar expression, shown in red) ahead of the wild-type differentiation front; arrow indicates an example of a precociously differentiated PR. (B and B′) Differentiation of PRs 3/4 (stained for Spalt expression, shown in red) is unaffected in tsc1Q87X clones. (C and D) Differentiation of PRs 1 and 6 (stained for Bar expression, shown in red in C and C′) and cone cells (stained with Cut, shown in red in D and D′) is strongly delayed in Rheb2D1 clones. (E and F) PR 2/5 (stained for Rough expression, shown in red in E and E′) and PR 3/4 (stained for Spalt expression, shown in red in F and F′) differentiation is unaffected in Rheb2D1 clones. LOF clones in all panels are marked by the loss of GFP staining (shown in green). Anterior is to the left in all panels.