Figure 4.—

pntP2 expression is regulated by InR/TOR signaling. (A and B) pntP2 transcription, detected by staining for β-galactosidase in flies carrying a P{LacW} element in pntP2 (pnt¹²⁷⁷), is upregulated and precocious in pten¹ (A and A′) and tsc2 (gig⁵⁶) clones (B and B′). Note that the disc shown in B is a younger disc and so pntP2 is upregulated more posteriorly. Conversely pntP2 transcription is downregulated in TOR^ΔD clones (C and C′). LOF clones in A–C are marked by the loss of GFP staining (shown in green). Anterior is to the left in all panels.